RING Finger Protein 10 Regulates AP-1/Meox2 to Mediate Pirarubicin-Induced Cardiomyocyte Apoptosis

作者全名："Shi, Hongwei; Duan, Liang; Lan, Ying; He, Quan; Pu, Peng; Tang, Heng"

作者地址："[Shi, Hongwei] Huazhong Univ Sci & Technol, Hubei Canc Hosp, Tongji Med Coll, Dept Radiat Oncol, Wuhan, Peoples R China; [Shi, Hongwei] Wuhan Univ, Dept Oncol, Renmin Hosp, Wuhan, Peoples R China; [Duan, Liang; Lan, Ying; He, Quan; Pu, Peng; Tang, Heng] Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiol, Chongqing, Peoples R China; [Tang, Heng] Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Cardiol, Chongqing 400038, Peoples R China"

通信作者："Pu, P; Tang, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiol, Chongqing, Peoples R China.; Tang, H (通讯作者)，Army Med Univ, Mil Med Univ 3, Southwest Hosp, Dept Cardiol, Chongqing 400038, Peoples R China."

来源：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000921557900003

JCR分区：Q2

影响因子：7.31

年份：2023

卷号：2023

期号：　

开始页：　

结束页：　

文献类型：Article

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摘要："Pirarubicin (THP) is one of the classic chemotherapy drugs for cancer treatment. It is often clinically limited because of its cardiotoxicity. The occurrence and development of THP-mediated chemotherapy-related cardiotoxicity (CRC) may be reversed by RING finger protein 10 (RNF10). This study was performed with the aim of evaluating the inhibitory effect of RNF10 on THP-mediated CRC and its molecular mechanism. In vivo, we found that the expression of RNF10 decreased in THP-induced CRC rats, accompanied by Meox2 inhibition and AP-1 activation, resulting in increased cardiomyocyte apoptosis. After small interfering RNA (siRNA) and lentivirus transfection (Lv) of RNF10 in vitro, the expression of RNF10, Meox2, and AP-1 proteins and the degree of cardiomyocyte apoptosis were detected. We found that overexpression of RNF10 in H9C2 cardiomyocytes significantly promoted Meox2 and inhibited AP-1, alleviated apoptosis, and showed further inhibitory activity on THP-induced cardiomyocyte toxicity. Silencing RNF10 showed the opposite result. Our study showed that RNF10 inhibited THP-induced CRC through the activity of Meox2 and AP-1 proteins. RNF10 may be the next drug target for the treatment of CRC and other related cardiovascular diseases."

基金机构：Chongqing Science and Health Joint Project [2020FYYX101]; Chinese Medicine Program of the Health Commission of Hubei Province [ZY2021Q003]; 7th Wuhan Young and Middle-aged Backbone Talent of Medical Training Project [87]

基金资助正文："The study was supported by the Chongqing Science and Health Joint Project (Grant number: 2020FYYX101), the Chinese Medicine Program of the Health Commission of Hubei Province (Grant number: ZY2021Q003), and the 7th Wuhan Young and Middle-aged Backbone Talent of Medical Training Project (Grant number: 2019 No. 87)."