Polysaccharides from aloe vera target the Wnt/13-catenin pathway to impact the tooth density of pulpitis rats

作者全名："Jiang, Ling; Lu, Yang; Zhao, Hongyan; He, Weiyang"

作者地址："[Jiang, Ling; He, Weiyang] Chongqing Med Univ, Affiliated Hosp Chongqing 1, Dept Urol Surg, Chongqing, Peoples R China; [Lu, Yang; Zhao, Hongyan] Chongqing Univ, Cent Hosp Chongqing, Dept Plast Surg, Chongqing, Peoples R China"

通信作者："He, WY (通讯作者)，Chongqing Med Univ, Affiliated Hosp Chongqing 1, Dept Urol Surg, Chongqing, Peoples R China."

来源：ACTA CIRURGICA BRASILEIRA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000922195500001

JCR分区：Q4

影响因子：1.1

年份：2022

卷号：37

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：Polysaccharides; Aloe; Pulpitis; Osteoblasts; Rats

摘要："Purpose: To investigate the mechanism of polysaccharides from aloe vera (PAV), a main active ingredient of Aloe vera, treatment in pulpitis rats. Methods: Pulpitis were modeled by drilling the occlusal central fossa with Sprague Dawley rats. Next, the rats were treated with 20, 40, and 80 mg/kg PAV for three weeks, respectively. Computed tomography scanning assay, hematoxylin and eosin staining, and tartrate-resistant acid phosphatase staining were used to detect the pathology change. Then, levels of tumor necrosis factor -a, interleukin-113, prostaglandin E2, and ciclooxigenase 2 were detected by enzyme-linked immunosorbent assay. The expressions of bone morphogenetic protein 2 human (BMP-2), osteocalcin, osterix, and runt-related transcription factor 2 (Runx2) were quantified by quantitative real-time polymerase chain reaction and Western blotting (WB). Finally, Wnt3a expression, p-GSK313/GSK313 and p-13- catenin/13-catenin ratio were analyzed by WB. Results: PAV up regulated the bone mineral density, and reduced the breakage of the crown and cervical structures, and the necrosis of the crown and root pulp of pulpitis rats. In addition, results indicated that PAV could inhibit osteoblast formation. While osteoblasts' number was decreased, proteins of BMP-2, osteocalcin, osterix, and Runx2 were up -regulated by PAV. Furthermore, PAV increased the Wnt3a expression and the p-13-catenin/13-catenin ratio, and decreased p-GSK313/ GSK313 ratio. Interestingly, these effects were all in dose dependence. Conclusions: PAV could inhibit pulp inflammation and promote osteoblasts differentiation via suppressing the activation of the Wnt/13-catenin signaling, enhancing the dental bone density."

基金机构："Chongqing Science and Health Joint Medical Research Project [2021MSXM131]; Basic Research and Frontier Exploration Project of Yuzhong District, Chongqing [20210178]"

基金资助正文："Chongqing Science and Health Joint Medical Research ProjectGrant No: 2021MSXM131Basic Research and Frontier Exploration Project of Yuzhong District, ChongqingGrant No: 20210178"