Isolation of Fusobacterium nucleatum from human feces using immunomagnetic separation coupled with fastidious anaerobe agar

作者全名："Tang, Jie; Dun, Guodong; Li, Jing; Xu, Qiaolin; Zhang, Tao; Lan, Yuanzhi; Zeng, Linghai; Luo, Huaxing; Xiang, Yuanyuan; Tang, Bin; Zeng, Dongzhu"

作者地址："[Tang, Jie; Li, Jing; Xu, Qiaolin; Zhang, Tao; Lan, Yuanzhi; Zeng, Linghai; Luo, Huaxing; Xiang, Yuanyuan; Tang, Bin; Zeng, Dongzhu] Chongqing Med Univ, Affiliated Hosp 3, Gener Hosp, Dept Gen Surg, Shuanghu Rd, Chongqing 401120, Peoples R China; [Dun, Guodong] 956th Hosp Chinese Peoples Liberat Army PLA, Dept Lab Med, Linzhi 860114, Peoples R China"

通信作者："Luo, HX; Xiang, YY; Tang, B; Zeng, DZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Gener Hosp, Dept Gen Surg, Shuanghu Rd, Chongqing 401120, Peoples R China."

来源：JOURNAL OF APPLIED MICROBIOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000922835500001

JCR分区：Q2

影响因子：3.2

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Fusobacterium nucleatum; immunomagnetic separation; immunomagnetic beads; feces

摘要："Aim Fusobacterium nucleatum (F. nucleatum) is associated with the initiation, development, and metastasis of colorectal cancer. However, it is difficult to isolate F. nucleatum from clinical specimens. In this study, we aimed to develop an effective and rapid method for isolating F. nucleatum from human feces using polyclonal antibody (PAB)-coated immunomagnetic beads (IMBs) with selective media. Methods and results IMBs conjugated with PAB were prepared and used to isolate F. nucleatum from human feces, and the bacteria were cultured with selective culture media (fastidious anaerobe agar + nalidixic acid + vancomycin). Under optimized experimental conditions, IMBs could selectively recover F. nucleatum from fecal microbiota samples spiked with Peptostreptococcus or Bacteroides fragilis. In artificial fecal samples, the detection sensitivity of IMBs for F. nucleatum was 10(3) CFU mL(-1). In addition, IMBs combined with selective media could rapidly isolate F. nucleatum from human feces. Conclusions This study successfully established an effective method for the rapid isolation of F. nucleatum from human feces by IMBs. The whole procedure requires 2-3 days, and has a sensitivity of 10(3) CFU mL(-1) feces."

基金机构："Science and Technology project of Yubei District of Chongqing [2020-35, 2021-37, 2022-28]"

基金资助正文："This work was supported by grants (Nos. 2020-35, 2021-37, and 2022-28) from the Science and Technology project of Yubei District of Chongqing."