CXCL12/CXCR4 Axis is Involved in the Recruitment of NK Cells by HMGB1 Contributing to Persistent Airway Inflammation and AHR During the Late Stage of RSV Infection

作者全名："Chen, Sisi; Tang, Wei; Yu, Guangyuan; Tang, Zhengzhen; Liu, Enmei"

作者地址："[Chen, Sisi] Chongqing Med & Pharmaceut Coll, Chongqing 401331, Peoples R China; [Chen, Sisi; Liu, Enmei] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp Med,Key Lab Child Dev & Disorders,Minist, Chongqing 400014, Peoples R China; [Tang, Wei] Univ Elect Sci & Technol China, Chengdu Womens & Childrens Cent Hosp, Sch Med, Resp Dept, Chengdu 610073, Peoples R China; [Yu, Guangyuan] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Dept Resp Med, Guangzhou 510623, Guangdong, Peoples R China; [Tang, Zhengzhen] Third Affiliated Hosp Med Univ, Peoples Hosp Zunyi 1, Dept Pediat, Zunyi 563000, Peoples R China"

通信作者："Liu, EM (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp Med,Key Lab Child Dev & Disorders,Minist, Chongqing 400014, Peoples R China."

来源：JOURNAL OF MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000929932700001

JCR分区：Q2

影响因子：3.3

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：RSV; HMGB1; NK cells; CXCL12; CXCR4

摘要："We previously showed that both high-mobility group box-1 (HMGB1) and natural killer (NK) cells contribute to respiratory syncytial virus (RSV)-induced persistent airway inflammation and airway hyperresponsiveness (AHR). Meanwhile, Chemokine (C-X-C motif) ligand 12 (CXCL12) and its specific receptor (chemokine receptor 4, CXCR4) play important roles in recruitment of immune cells. CXCL12 has been reported to form a complex with HMGB1 that binds to CXCR4 and increases inflammatory cell migration. The relationship between HMGB1, NK cells and chemokines in RSV-infected model remains unclear. An anti-HMGB1 neutralizing antibody and inhibitor of CXCR4 (AMD3100) was administered to observe changes of NK cells and airway disorders in nude mice and BALB/c mice. Results showed that the mRNA expression and protein levels of HMGB1 were elevated in late stage of RSV infection and persistent airway inflammation and AHR were diminished after administration of anti-HMGB1 antibodies, with an associated significant decrease in CXCR4(+) NK cells. In addition, CXCL12 and CXCR4 were reduced after HMGB1 blockade. Treatment with AMD3100 significantly suppressed the recruitment of NK cells and alleviated the airway disorders. Thus, CXCL12/CXCR4 axis is involved in the recruitment of NK cells by HMGB1, contributing to persistent airway inflammation and AHR during the late stage of RSV infection."

基金机构："Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0303]; National Natural Science Foundation of China [81900008, 82200011]; Science and Technology Project of Guizhou Province; Project of Chongqing Medical and Pharmaceutical College [Qianke Foundation [2019]1327]; [ygz2022112]"

基金资助正文："This work was supported by the Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0303), National Natural Science Foundation of China (81900008, 82200011), Science and Technology Project of Guizhou Province (No. Qianke Foundation [2019]1327) and Project of Chongqing Medical and Pharmaceutical College (No. ygz2022112)."