Liposome-trimethyl chitosan nanoparticles codeliver insulin and siVEGF to treat corneal alkali burns by inhibiting ferroptosis

作者全名："Xiong, Xiaojing; Jiang, Huiting; Liao, Yukun; Du, Yangrui; Zhang, Yu; Wang, Zhigang; Zheng, Minming; Du, Zhiyu"

作者地址："[Xiong, Xiaojing; Jiang, Huiting; Liao, Yukun; Du, Yangrui; Zhang, Yu; Zheng, Minming; Du, Zhiyu] Chongqing Med Univ, Affiliated Hosp 2, Dept Ophthalmol, Chongqing 400010, Peoples R China; [Xiong, Xiaojing; Jiang, Huiting; Liao, Yukun; Wang, Zhigang] Chongqing Med Univ, Affiliated Hosp 2, Chongqing Key Lab Ultrasound Mol Imaging, Chongqing, Peoples R China; [Xiong, Xiaojing] Chongqing Med Univ, Affiliated Hosp 2, State Key Lab Ultrasound Med & Engn, Chongqing, Peoples R China"

通信作者："Du, ZY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Ophthalmol, Chongqing 400010, Peoples R China."

来源：BIOENGINEERING & TRANSLATIONAL MEDICINE

ESI学科分类：Multidisciplinary

WOS号：WOS:000931722300001

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：8

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：corneal alkali burn; ferroptosis inhibition; insulin; nanomedicine; siVEGF

摘要："Alkali burns are potentially blinding corneal injuries. Due to the lack of available effective therapies, the prognosis is poor. Thus, effective treatment methods for corneal alkali burns are urgently needed. Codelivery nanoparticles (NPs) with characteristics such as high bioavailability and few side effects have been considered effective therapeutic agents for ocular diseases. In this study, we designed a new combination therapy using liposomes and trimethyl chitosan (TMC) for the codelivery of insulin (INS) and vascular endothelial growth factor small interfering RNA (siVEGF) to treat alkali-burned corneas. We describe the preparation and characterization of siVEGF-TMC-INS-liposome (siVEGF-TIL), drug release characteristics, intraocular tracing, pharmacodynamics, and biosafety. We found that siVEGF-TIL could inhibit oxidative stress, inflammation, and the expression of VEGF in vitro and effectively maintained corneal transparency, accelerated epithelialization, and inhibited corneal neovascularization (CNV) in vivo. Morever, we found that the therapeutic mechanism of siVEGF-TIL is possibly relevant to the inhibition of the ferroptosis signaling pathway by metabolomic analysis. In general, siVEGF-TIL NPs could be a safe and effective therapy for corneal alkali burn."

基金机构：Key Program of Chongqing Natural Science Foundation [cstc2021ycjh-bgzxm0064]

基金资助正文："Key Program of Chongqing Natural Science Foundation, Grant/Award Number: cstc2021ycjh-bgzxm0064"