Transcriptional activation of CSTB gene expression by transcription factor Sp3

作者全名："Zhu, Weiyi; Dong, Xiangjun; Luo, Shuyue; Guo, Shipeng; Zhou, Weihui; Song, Weihong"

作者地址："[Zhu, Weiyi; Dong, Xiangjun; Luo, Shuyue; Guo, Shipeng; Zhou, Weihui; Song, Weihong] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Inst Aging, Zhejiang Prov Clin Res Ctr Mental Disorders, Sch Mental Hlth,Key Lab Alzheimers Dis Zhejiang Pr, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Kangning Hosp, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Oujiang Lab, Zhejiang Lab Regenerat Med Vision & Brain Hlth, Wenzhou 325001, Zhejiang, Peoples R China"

通信作者："Zhou, WH; Song, WH (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000931782300001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：649

期号：　

开始页：71

结束页：78

文献类型：Article

关键词：CSTB; Sp3; Transcriptional regulation; Hepatocellular carcinoma

摘要："CSTB has been reported to be associated with the pathogenesis of many malignant tumors, especially hepatocellular carcinoma (HCC). However, how the expression of this gene is regulated is largely un-known. We initially cloned and analyzed the promoter region of the CSTB gene by luciferase assay and the Sp3 binding site (CCCCGCCCCGCG) was found in it. The results of electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) experiments verified that the transcription factor, Sp3 could bind to the "" CCCCGCCCCGCG 00 site of the CSTB gene promoter. We showed that the over-expression of Sp3 significantly increased the endogenous mRNA and protein expression levels of CSTB, whereas knockdown of Sp3 decreased the mRNA and protein expression levels according to quantitative real-time PCR (qRT-PCR) and western blotting. In conclusion, CSTB gene expression is closely regulated by transcription factor Sp3, which may be a potential mechanism for the dysregulation of CSTB expression in HCC.(c) 2023 Elsevier Inc. All rights reserved."

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