Generation of a NIS-EGFP-Fluc triple-reporter human embryonic stem cell line by PiggyBac transposon system

作者全名："Huang, Pufeng; Tian, Chuanhuizi; Chen, Yanyan; Zhou, Meng; Ni, Jie; Niu, Mengda; Liu, Xujie; Peng, Zhiping"

作者地址："[Huang, Pufeng; Tian, Chuanhuizi; Chen, Yanyan; Niu, Mengda; Peng, Zhiping] Chongqing Med Univ, Coll Basic Med, Dept Radiol Med & Oncol, Chongqing 400016, Peoples R China; [Huang, Pufeng; Zhou, Meng; Ni, Jie; Liu, Xujie] Chinese Acad Med Sci, Shenzhen Key Lab Cardiovasc Dis, Fuwai Hosp, Shenzhen 518057, Guangdong, Peoples R China"

通信作者："Peng, ZP (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Radiol Med & Oncol, Chongqing 400016, Peoples R China.; Liu, XJ (通讯作者)，Chinese Acad Med Sci, Shenzhen Key Lab Cardiovasc Dis, Fuwai Hosp, Shenzhen 518057, Guangdong, Peoples R China."

来源：STEM CELL RESEARCH

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000933957000003

JCR分区：Q4

影响因子：1.2

年份：2022

卷号：65

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："One major challenge in stem cell therapy is to longitudinally track cell fate after cells transplantation. Molecular Imaging approaches enabling noninvasive long-term monitoring the transplanted cells are imperative for assessment of the safety and efficiency. Here, we used PiggyBac technology to insert triple reporter genes: NIS, EGFP and Firefly luciferase into a human embryonic stem cell line (hESCs, H9) and obtained a reporter hESCs line (NIS-EGFP-Fluc H9). The triple-reporters allows the transplanted NIS-EGFP-Fluc H9 cells and their derivates to be fluorescence, bioluminescence and even PET/SPECT imaged. This triple-reporter hESCs line provides a valuable imaging platform for cell-based therapeutics clinical translation."

基金机构：National Natural Science Foundation of China [81771871]; Shenzhen Fundamental Research Program [JCYJ20220531091615034]; Shenzhen High-level Hospital Construction Fund

基金资助正文："This work was supported by the National Natural Science Foundation of China (81771871), Shenzhen Fundamental Research Program (JCYJ20220531091615034) and Shenzhen High-level Hospital Construction Fund."