"Genetic and enzymatic characterization of two novel bla(NDM-36,-37) variants in Escherichia coli strains"

作者全名："Ma, Wanshan; Zhu, Bo; Wang, Wen; Wang, Qian; Cui, Xiaodi; Wang, Yujiao; Dong, Xiutao; Li, Xiaofeng; Ma, Jianping; Cheng, Fang; Shi, Xiaohong; Chen, Liang; Niu, Siqiang; Hao, Mingju"

作者地址："[Ma, Wanshan; Wang, Yujiao; Dong, Xiutao; Li, Xiaofeng; Ma, Jianping; Cheng, Fang; Shi, Xiaohong; Hao, Mingju] Shandong First Med Univ, Dept Clin Lab Med, Affiliated Hosp 1, 16766 Jingshi Rd, Jinan, Peoples R China; [Ma, Wanshan; Wang, Yujiao; Dong, Xiutao; Li, Xiaofeng; Ma, Jianping; Cheng, Fang; Shi, Xiaohong; Hao, Mingju] Shandong Prov Qianfoshan Hosp, Shandong Med & Hlth Key Lab Lab Med, 16766 Jingshi Rd, Jinan, Peoples R China; [Zhu, Bo] Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen, Peoples R China; [Wang, Wen; Niu, Siqiang] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 1, 1 Friendship Rd, Chongqing, Peoples R China; [Wang, Qian] Shandong First Med Univ & Shandong Acad Med Sci, Dept Lab Med, Shandong Prov Hosp, Jinan, Shandong, Peoples R China; [Cui, Xiaodi] Jining Med Univ, Sch Clin Med, Jining, Peoples R China; [Chen, Liang] Hackensack Meridian Hlth, Ctr Discovery & Innovat, Nutley, NJ USA; [Chen, Liang] Hackensack Meridian Sch Med, Dept Med Sci, Nutley, NJ USA"

通信作者："Hao, MJ (通讯作者)，Shandong First Med Univ, Dept Clin Lab Med, Affiliated Hosp 1, 16766 Jingshi Rd, Jinan, Peoples R China.; Hao, MJ (通讯作者)，Shandong Prov Qianfoshan Hosp, Shandong Med & Hlth Key Lab Lab Med, 16766 Jingshi Rd, Jinan, Peoples R China.; Niu, SQ (通讯作者)，Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 1, 1 Friendship Rd, Chongqing, Peoples R China."

来源：EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000936953500001

JCR分区：Q2

影响因子：3.7

年份：2023

卷号：42

期号：4

开始页：471

结束页：480

文献类型：Article

关键词：Carbapenems; E; coli; Bla(NDM-36); Bla(NDM-37); Urinary tract infection

摘要："The widespread of different NDM variants in clinical Enterobacterales isolates poses a serious public health concern, which requires continuous monitoring. In this study, three E. coli strains carrying two novel bla(NDM) variants of bla(NDM-36, -37) were identified from a patient with refractory urinary tract infection (UTI) in China. We conducted antimicrobial susceptibility testing (AST), enzyme kinetics analysis, conjugation experiment, whole-genome sequencing (WGS), and bioinformatics analysis to characterize the bla(NDM-36, -37) enzymes and their carrying strains. The bla(NDM-36, -37) harboring E. coli isolates belonged to ST227, O9:H10 serotype and exhibited intermediate or resistance to all beta-lactams tested except aztreonam and aztreonam/avibactam. The genes of bla(NDM-36, -37) were located on a conjugative IncHI2-type plasmid. NDM-37 differed from NDM-5 by a single amino acid substitution (His261Tyr). NDM-36 differed from NDM-37 by an additional missense mutation (Ala233Val). NDM-36 had increased hydrolytic activity toward ampicillin and cefotaxime relative to NDM-37 and NDM-5, while NDM-37 and NDM-36 had lower catalytic activity toward imipenem but higher activity against meropenem in comparison to NDM-5. This is the first report of co-occurrence of two novel bla(NDM) variants in E. coli isolated from the same patient. The work provides insights into the enzymatic function and demonstrates the ongoing evolution of NDM enzymes."

基金机构："Shandong Provincial Natural Science Foundation [ZR2021MH078]; Clinical amp; Medical Science and Technology Innovation Program of Jinan, Shandong Province [202134040]; Cultivate Fund from The First Affiliated Hospital of Shandong First Medical University amp; Shandong Provincial Qianfoshan Hospital [QYPY2022NSFC0802, 2020NSFC0808]"

基金资助正文："This work was supported by the Shandong Provincial Natural Science Foundation [Grant number ZR2021MH078]; Clinical & Medical Science and Technology Innovation Program of Jinan, Shandong Province [Grant number 202134040]; and Cultivate Fund from The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital [Grant number QYPY2022NSFC0802, 2020NSFC0808]. The above funding bodies play a role in the design of the study and collection, analysis, and interpretation of data."