An optimized method for Oil Red O staining with the salicylic acid ethanol solution

作者全名："Du, Junbao; Zhao, Li; Kang, Quan; He, Yun; Bi, Yang"

作者地址："[Du, Junbao; Zhao, Li; Kang, Quan; He, Yun; Bi, Yang] Chongqing Med Univ, Stem Cell Biol & Therapy Lab, Childrens Hosp, Chongqing, Peoples R China; [Du, Junbao; Zhao, Li; He, Yun; Bi, Yang] Chongqing Key Lab Pediat, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Bi, Yang] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Stem Cell Biol & Therapy Lab, Minist Educ Key Lab Child Dev & Disorders,Children, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China"

通信作者："Bi, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Stem Cell Biol & Therapy Lab, Minist Educ Key Lab Child Dev & Disorders,Children, Bldg 7,Room 905,136 Zhongshan Er Rd, Chongqing 400014, Peoples R China."

来源：ADIPOCYTE

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000938578200001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Oil red O staining; ethanol; salicylic acid; isopropanol; lipid droplet

摘要："Oil Red O (ORO) staining is a commonly used experimental technique to detect lipid content in cells or tissues. Freshly prepared ORO in 60% isopropanol is the most widely used method at present. However, isopropanol is volatile and harmful to the human body. It will also affect the interpretation of the results due to the formation of crystals and non-specific diffuse staining. In this paper, by screening and validation, we report a salicylic acid ethanol solution (containing 50% ethanol, 5%-10% salicylic acid) for the preparation of ORO solution, which has a better staining effect on lipid staining in cells and tissues, with a clean background and short dyeing time. What's more, this ORO solution is non-toxic, convenient to prepare, and can be stored for a long time. Therefore, it is reliable, easy to operate, and can be widely popularized and applied in laboratories."

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