Self-supply of H2O2 and O2 by a composite nanogenerator for chemodynamic therapy/hypoxia improvement and rapid therapy of biofilm-infected wounds

作者全名："Wu, Junjie; Shen, Peng; Qin, Xian; Yang, Yulu; Lin, Chuanchuan; Li, Xuan; Geng, Wenbo; Gao, Pengfei; Chen, Lixue; Miao, Li; Jiao, Yang; Tao, Bailong"

作者地址："[Wu, Junjie; Chen, Lixue; Tao, Bailong] Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400016, Peoples R China; [Shen, Peng] Peoples Liberat Army Gen Hosp, Dept Gen Internal Med, Northern Med Branch, Beijing 100094, Peoples R China; [Qin, Xian] Chongqing Hlth Ctr Women & Children, Dept Reprod Endocrinol, Chongqing 401147, Peoples R China; [Yang, Yulu; Li, Xuan; Geng, Wenbo; Gao, Pengfei] Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China; [Lin, Chuanchuan] Third Mil Med Univ, Affiliated Hosp 2, Dept Blood Transfus, Lab Radiat Biol, Chongqing 400037, Peoples R China; [Miao, Li; Jiao, Yang] Peoples Liberat Army Gen Hosp, Med Ctr 7, Dept Stomatol, Beijing 100700, Peoples R China"

通信作者："Tao, BL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Lab Res Ctr, Chongqing 400016, Peoples R China.; Miao, L; Jiao, Y (通讯作者)，Peoples Liberat Army Gen Hosp, Med Ctr 7, Dept Stomatol, Beijing 100700, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000942578600001

JCR分区：Q1

影响因子：13.3

年份：2023

卷号：459

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Chemodynamic therapy; Hypoxia; Zeolitic imidazolate frameworks-67; Antibofilm capatibility; Wound healing; pH-responsiveness

摘要："Bacterial wound infection is a major challenge to human health worldwide. In this study, a composite nano -generator, CaO2@ZIF-67-PLL, was fabricated via a bottom-up approach with chemodynamic therapy (CDT)/ hypoxia improvement for rapid therapy of biofilm-infected wounds. Under a pathologically acidic microenvi-ronment, the out layer ZIF-67 can be decomposed to rapidly release Co2+ and CaO2; Subsequently, the unpro-tected CaO2 reacts with H2O to produce O2 and H2O2. The self-supply of O2 relieves hypoxia and inflammatory response induced by infections, while the generated H2O2 and poly -L-lysine (PLL) exhibited synergistic strong anti-biofilm capability against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as assessed by reactive oxygen species (ROS) generation, membrane permeability change, ATP reduction, o-nitrophenyl-beta-d-galactopyranoside (ONPG) hydrolysis and intracellular component leakage. Meanwhile, CaO2@ZIF-67-PLL exhibited good cytocompatibility and promoted the adhesion, proliferation, and migration of NIH-3T3 cells by enhancing the expression of wound healing-related genes (TGF-beta 1, VEGF, alpha-SMA, Col I, Col III, and Wnt-5a). Notably, CaO2@ZIF-67-PLL could significantly improve the healing process of S. aureus-infected wounds via suppressed inflammatory responses, enhanced collagen disposition and angiogenesis, and stimulated HIF-1 alpha, CD31, and Wnt-5a/beta-Catenin signaling pathways. These results indicated the potential of CaO2@ZIF-67-PLL nanogenerator for clinical application in infected wound treatment."

基金机构："National Natural Science Foundation of China [82101069, 82102537]; Natural Science Foundation of Chongqing Science and Technology Commission [CSTC2021JCYJ-MSXMX0170, CSTB2022BSXM-JCX0039]; Chongqing Medical University Cultivating Fund [PYJJ2021-02, PYJJ2021-04]; Beijing Municipal Science & Technology Commission [Z221100007422130]; Youth In-cubation Program of Medical Science and Technology of PLA [21QNPY116]"

基金资助正文："This work was financially supported by National Natural Science Foundation of China (No. 82101069 & 82102537) , Natural Science Foundation of Chongqing Science and Technology Commission (No. CSTC2021JCYJ-MSXMX0170 & CSTB2022BSXM-JCX0039) , the First Affiliated Hospital of Chongqing Medical University Cultivating Fund (No. PYJJ2021-02 & PYJJ2021-04) , Beijing Municipal Science & Technology Commission (No. Z221100007422130) , and the Youth In-cubation Program of Medical Science and Technology of PLA (No. 21QNPY116) ."