iTRAQ and PRM-Based Comparative Proteomic Profiling of the Hippocampus in Rat Models of Epilepsy

作者全名："Zhao, Congcong; Zheng, Zhe; Zhu, Shisheng; Zhang, Li; Zhang, Peng; Xiong, Hongli; Li, Yongguo; Zhu, Ying; Zhao, Minzhu; Li, Jianbo"

作者地址："[Zhao, Congcong; Zheng, Zhe; Zhang, Li; Zhang, Peng; Xiong, Hongli; Li, Yongguo; Zhu, Ying; Zhao, Minzhu; Li, Jianbo] Chongqing Med Univ, Dept Forens Med, Chongqing 400010, Peoples R China; [Zhu, Shisheng] Chongqing Med & Pharmaceut Coll, Fac Med Technol, Chongqing 401331, Peoples R China; [Zhu, Shisheng] Chongqing Engn Res Ctr Pharmaceut Sci, Chongqing 401331, Peoples R China"

通信作者："Zhao, MZ; Li, JB (通讯作者)，Chongqing Med Univ, Dept Forens Med, Chongqing 400010, Peoples R China."

来源：JOURNAL OF INTEGRATIVE NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000942963600001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：epilepsy; hippocampus; proteomic; iTRAQ; PRM

摘要："Background: Epilepsy is a disease caused by paroxysmal abnormal supersynchronous electrical activity of brain neurons, and it is also one of the most common illnesses in neurology. Among the causes, hippocampal sclerosis may be one of the main causes of temporal lobe epilepsy. However, the pathogenesis of hippocampal sclerosis in epilepsy remains unclear. Methods: We established an epilepsy model by intraperitoneal injection of pentetrazol (PTZ) into Sprague-Dawley rats, and applied isobaric tags for relative and absolute quantitation (iTRAQ) technology to identify differentially expressed proteins (DEPs) in the hippocampus. We quantified a total of 3782 proteins. DEPs were defined as proteins with a fold change >1.2 (or <0.83) and a Q value (p-adjusted) <0.05. Results: Comparing the epilepsy group and the control group, we identified 170 DEPs, comprising 109 upregulated and 61 downregulated proteins. According to bioinformatics analysis, the DEPs were primarily involved in long-term potentiation, the calcium signalling pathway, aldosterone synthesis and secretion, carbon metabolism, and dopaminergic synapses. Four of these proteins were validated using parallel reaction monitoring (PRM), including Glud1, Atp1a2, Prkcg and Arpc3. Conclusions: Our research results may provide further insight into the molecular pathology of hippocampal injury in epilepsy."

基金机构："Basic and Frontier Technological Research Program of Chongqing [cstc2019jcyj-msxmX0455]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201902804, KJQN201902805]; Scientific and Technological Research Program of Chongqing Medical and Pharmaceutical College [ygz2018103]; Innovative Research Groups of Chongqing Medical and Pharmaceutical College [YGZ2019402]; postdoctoral Science Fund Project of Chongqing Natural Science Foundation [cstc2019jcyj-bshX0128]; National Natural Science Foundation of China [81901922]"

基金资助正文："The research was funded by the Basic and Frontier Technological Research Program of Chongqing (cstc2019jcyj-msxmX0455), the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201902804; KJQN201902805), the Scientific and Technological Research Program of Chongqing Medical and Pharmaceutical College (ygz2018103), the Innovative Research Groups of Chongqing Medical and Pharmaceutical College (YGZ2019402), postdoctoral Science Fund Project of Chongqing Natural Science Foundation (cstc2019jcyj-bshX0128), the National Natural Science Foundation of China (81901922)."