Primer Exchange Reaction Coupled with DNA-Templated Silver Nanoclusters for Label-Free and Sensitive Detection of MicroRNA

作者全名："Ning, Linhong; Li, Yuanhong; Zhang, Zhimei; Zhou, Yuan; Yang, Li; Yu, Qinghai; Yu, Feng; Tong, Zehui"

作者地址："[Ning, Linhong; Zhou, Yuan; Yu, Qinghai] Coll Chongqing Med & Pharmaceut Coll, Chongqing 401331, Peoples R China; [Li, Yuanhong] Chongqing Med Univ, Dept Gastroenterol, Chongqing Jiangjin Dist Cent Hosp, Chongqing 402260, Peoples R China; [Zhang, Zhimei] Chongqing Med Univ, Dept Gastroenterol, Banan Dist Peoples Hosp, Chongqing 401320, Peoples R China; [Yang, Li; Tong, Zehui] Chongqing Univ, Chongqing Shapingba Dist Peoples Hosp, Dept Gastroenterol, Chongqing 400055, Peoples R China; [Yu, Feng] Chenjiaqiao Hosp, Chongqing Med & Pharmaceut Coll, Dept Gastroenterol, Chongqing 401331, Peoples R China"

通信作者："Tong, ZH (通讯作者)，Chongqing Univ, Chongqing Shapingba Dist Peoples Hosp, Dept Gastroenterol, Chongqing 400055, Peoples R China.; Yu, F (通讯作者)，Chenjiaqiao Hosp, Chongqing Med & Pharmaceut Coll, Dept Gastroenterol, Chongqing 401331, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000942974800003

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Primer exchange reaction (PER); DNA template; MicroRNAs; Silver nanoclusters; Pancreatitis

摘要："MicroRNAs (MiRNAs) play pivotal roles in regulating gene expression, and serve as crucial biomarkers for diagnosis of a variety of disease. However, label-free and sensitive miRNA detection remains a huge challenge due to the low abundance. Herein, we developed an approach through integrating primer exchange reaction (PER) with DNA-templated silver nanoclusters (AgNCs) for label-free and sensitive miRNA detection. In this method, PER was used to amplify miRNA signals and produce single-strand DNA (ssDNA) sequences. The produced ssDNA sequences mediated DNA-templated AgNCs based signal generation by unfolding the designed hairpin probe (HP). The generated AgNCs signal was correlated with the dosage of target miRNA. Eventually, the established approach exhibited a low detection of limit of 47 fM with a great dynamic range of more than five orders of magnitude. In addition, the method was also utilized to detect the miRNA-31 expression in collected clinical samples from pancreatitis patients and demonstrated that miRNA-31 was upregulated in patients, showing a great promising of the method in clinical application."

基金机构：Natural Science Foundation of Chongqing Science and Technology Commission [cstc2021jcyj-msxmX0916]; Natural Science Foundation of Chongqing Medical and Pharmaceutical College [ygz2019302]

基金资助正文：AcknowledgementsWe appreciate the financial support from the Natural Science Foundation of Chongqing Science and Technology Commission (cstc2021jcyj-msxmX0916) and the Natural Science Foundation of Chongqing Medical and Pharmaceutical College (ygz2019302). We also thank the director of central laboratory for providing essential equipment.