A hairpin probe-mediated exponential amplification reaction for highly sensitive and specific detection of microRNAs

作者全名："Yu, Hongyan; Weng, Zhi; Zhou, Xi; Bai, Dan; Luo, Wang; Han, Xiaole; Song, Lin; Liu, Qian; Li, Junjie; Yang, Yujun; Guo, Yongcan; Lv, Ke; Xie, Guoming"

作者地址："[Yu, Hongyan; Weng, Zhi; Zhou, Xi; Bai, Dan; Luo, Wang; Han, Xiaole; Song, Lin; Liu, Qian; Li, Junjie; Yang, Yujun; Xie, Guoming] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Yu, Hongyan; Weng, Zhi; Zhou, Xi; Bai, Dan; Luo, Wang; Han, Xiaole; Song, Lin; Liu, Qian; Li, Junjie; Yang, Yujun; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Lv, Ke] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China; [Guo, Yongcan] Southwest Med Univ, LuZhou Key Lab Nanobiosensing & Microfluid Point o, Chinese Med Hosp, Clin Lab Tradit, Luzhou 646000, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing Med Lab Microfluid, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Lv, K (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China.; Guo, YC (通讯作者)，Southwest Med Univ, LuZhou Key Lab Nanobiosensing & Microfluid Point o, Chinese Med Hosp, Clin Lab Tradit, Luzhou 646000, Peoples R China."

来源：CHEMICAL COMMUNICATIONS

ESI学科分类：CHEMISTRY

WOS号：WOS:000944197700001

JCR分区：Q2

影响因子：4.9

年份：2023

卷号：59

期号：28

开始页：4158

结束页：4161

文献类型：Article

关键词：　

摘要："In this work, we propose a hairpin probe-mediated exponential amplification reaction (HEAR) strategy that combines DNA strand displacement with a ""who triggers, who gets generated"" mode, providing excellent single-base discrimination and a reduced background signal. The detection limit is 19 aM, which is reduced by 3 orders of magnitude compared to traditional exponential amplification approaches. This one-pot strategy also exhibits a wide dynamic range, high specificity and short detection time. It is expected to become a powerful tool for clinical diagnosis."

基金机构：National key R&D Program of China [2022YFC2603803]; National Natural Science Foundation of China [82172369]

基金资助正文：This work was financially supported by the National key R&D Program of China (2022YFC2603803) and the National Natural Science Foundation of China (82172369).