The anti-hepatocellular carcinoma effects of polysaccharides from Ganoderma lucidum by regulating macrophage polarization via the MAPK/NF-kappa B signaling pathway

作者全名："Li, Guo-li; Tang, Jia-feng; Tan, Wen-li; Zhang, Tao; Zeng, Di; Zhao, Shuang; Ran, Jian-hua; Li, Jing; Wang, Ya-ping; Chen, Di-long"

作者地址："[Li, Guo-li; Tang, Jia-feng; Zeng, Di; Zhao, Shuang; Li, Jing; Wang, Ya-ping; Chen, Di-long] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China; [Zhang, Tao; Ran, Jian-hua] Chongqing Med Univ, Coll Basic Med, Neurosci Res Ctr, Chongqing, Peoples R China; [Li, Guo-li; Tang, Jia-feng; Tan, Wen-li; Zhang, Tao; Chen, Di-long] Chongqing Three Gorges Med Coll, Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, Chongqing, Peoples R China"

通信作者："Wang, YP; Chen, DL (通讯作者)，Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China.; Chen, DL (通讯作者)，Chongqing Three Gorges Med Coll, Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, Chongqing, Peoples R China."

来源：FOOD & FUNCTION

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:000945410300001

JCR分区：Q1

影响因子：5.1

年份：2023

卷号：14

期号：7

开始页：3155

结束页：3168

文献类型：Article

关键词：　

摘要："The response of macrophages to environmental signals demonstrates its heterogeneity and plasticity. After different forms of polarized activation, macrophages reach the M1 or M2 activation state according to their respective environment. Ganoderma lucidum polysaccharide (GLPS) is a major bioactive component of Ganoderma lucidum, a well-known medicinal mushroom. Although the immunomodulatory and anti-tumor effects of GLPS have been proven, GLPS's effect on inhibiting hepatocellular carcinoma (HCC) by regulating macrophage polarization is little known. Our data showed that GLPS notably inhibited the growth of a Hepa1-6 allograft. The expression of M1 marker CD86 was higher in the tumor tissue of the GLPS treatment group than in the control group in vivo. In vitro, the phagocytic activity and NO production of macrophages were increased by GLPS treatment. Moreover, it was discovered that GLPS was able to increase the expression of the M1 phenotype marker CD86, iNOS, and pro-inflammatory cytokines comprising IL-12a, IL-23a, IL-27 and TNF-alpha, but inhibited macrophage polarization towards the M2 phenotype by decreasing the expression of CD206, Arg-1, and inflammation-related cytokines comprising IL-6 and IL-10. The data suggest that GLPS may regulate macrophage polarization. Mechanistically, GLPS increased the phosphorylation of MEK and ERK. In addition, the phosphorylation of I kappa B alpha and P65 was increased by GLPS treatment. These data showed that GLPS can regulate the MAPK/NF-kappa B signaling pathway responsible for M1 polarization. In a nutshell, our research puts forward a new application of GLPS in anti-HCC treatment by regulating macrophage polarization through activating MAPK/NF-kappa B signaling."

基金机构："Key Project and a Youth Project of the Science and Technology Research Program of the Chongqing Education Commission of China [KJZD-K201802701, KJQN202002710]; Key Project and a Lab Project of Chongqing Three Gorges Medical College of China [2019XZZ002, SYS20210005]"

基金资助正文："This study was supported by a Key Project and a Youth Project of the Science and Technology Research Program of the Chongqing Education Commission of China (No. KJZD-K201802701 and KJQN202002710), a Key Project and a Lab Project of Chongqing Three Gorges Medical College of China (No. 2019XZZ002 and SYS20210005)."