The Combination of JAK2V617F Allele Burden and WT1 Expression can Be Helpful in Distinguishing the Subtype of MPN Patients

作者全名："Wu, Sanyun; Luo, Ping; Rouzi, Tuerxunayi; Yu, Yalan; Xiong, Bei; Wang, Yingying; Zuo, Xuelan"

作者地址："[Wu, Sanyun; Luo, Ping; Rouzi, Tuerxunayi; Yu, Yalan; Xiong, Bei; Zuo, Xuelan] Zhongnan Hosp Wuhan Univ, Dept Hematol, Wuhan, Peoples R China; [Wang, Yingying] Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing, Peoples R China; [Zuo, Xuelan] Wuhan Univ, Zhongnan Hosp, Dept Hematol, Donghu Rd 169, Wuhan 430071, Peoples R China"

通信作者："Zuo, XL (通讯作者)，Wuhan Univ, Zhongnan Hosp, Dept Hematol, Donghu Rd 169, Wuhan 430071, Peoples R China."

来源：CANCER CONTROL

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000946557300001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：30

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：WT1; MPN; ET; PV; PMF

摘要："IntroductionClassical Philadelphia-negative myeloproliferative neoplasm (MPN) includes Essential Thrombocythemia (ET), Polycythemia Vera (PV) and Primary Myelofibrosis (PMF). The JAK2V617F mutation is part of the major criteria for diagnosis of MPN. WT1 is reported to be highly overexpressed in most hematological malignancy. Our aim was to explore the combination value of JAK2V617F allele burden and WT1 expression in distinguishing the subtype of MPN patients.MethodsAllele specific real-time quantitative fluorescence PCR (AS-qPCR) was conducted to detect JAK2V617F allele burden. WT1 expression was assessed by RQ-PCR. Our study is a retrospective study.ResultsJAK2V617F allele burden and WT1 expression were different in MPN subgroups. The expression of WT1 in PMF and PV is higher than in ET. JAK2V617F allele burden in PMF and PV is also higher than in ET. ROC analysis indicated that combination of JAK2V617F allele burden and WT1 expression to discriminate ET and PV, ET and PMF, PV and PMF is 0.956, 0.871, 0.737 respectively. Furthermore, their ability to distinguish ET patients with high Hb levels from PV patients with high platelet counts is 0.891.ConclusionsOur data revealed that combination of JAK2V617F allele burden and WT1 expression is useful in distinguishing the subtype of MPN patients."

基金机构：National Natural Science Foundation of China [81670125]; Natural Science Foundation of Hubei Province [2018CFB243]

基金资助正文："The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by National Natural Science Foundation of China (81670125), Natural Science Foundation of Hubei Province (2018CFB243)."