Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients

作者全名："Chang, Luyue; Wu, Haiping; Chen, Rui; Sun, Xiaoqing; Yang, Yi; Huang, Changwu; Ding, Shijia; Liu, Changjin; Cheng, Wei"

作者地址："[Chang, Luyue; Liu, Changjin; Cheng, Wei] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China; [Sun, Xiaoqing; Yang, Yi; Huang, Changwu; Liu, Changjin] Fifth Peoples Hosp Chongqing, Dept Lab Med, Chongqing 400062, Peoples R China; [Wu, Haiping; Chen, Rui; Ding, Shijia] Chongqing Med Univ, Minist Educ, Coll Lab Med, Key Lab Clin Lab Diagnost, Chongqing 400016, Peoples R China"

通信作者："Liu, CJ; Cheng, W (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China.; Liu, CJ (通讯作者)，Fifth Peoples Hosp Chongqing, Dept Lab Med, Chongqing 400062, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000948388700001

JCR分区：Q1

影响因子：10.6

年份：2023

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：PD-L1(+) exosome; Electrochemical aptasensor; Microporous PdCuB nanotag; Au@CuCl2 nanowires; Non-small cell lung cancer

摘要："Programmed cell death ligand 1 protein-positive (PD-L1(+)) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1(+) exosomes is still a challenge in clinical applications. Herein, a sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl2 nanowires (NWs) was designed for the detection of PD-L1(+) exosomes. The excellent peroxidase-like catalytic activity of PdCuB MNs and the high conductivity of Au@CuCl2 NWs endow the fabricated aptasensor with intense electrochemical signal, thus enabling the detection of low abundance exosomes. The analytical results revealed that the aptasensor maintained favorable linearity over a wide concentration range of 6 orders of magnitude and reached a low detection limit of 36 particles/mL. The aptasensor is successfully applied to the analysis of complex serum samples and achieves the accurate identification of clinical NSCLC patients. Overall, the developed electrochemical aptasensor provides a powerful tool for early diagnosis of NSCLC."

基金机构："National Natural Science Foundation of China [81873972, 81873980]; Chongqing Science Fund for Distinguished Young Scholars [Qiankehejichu-ZK [2022] 654]; Science and Technology Project of Guizhou [Qiankehejichu-ZK [2022] 654]; Science and Technology Fund of Guizhou Health Commission [gzwkj2022-175]; Chongqing Science Fund for Distinguished Young Scholars [cstc2019j-cyjjqX0028]; Foundation for Innovative Research Groups of Chongqing Higher Education Institutions [CXQT20013]; Chongqing Talents-Innovation Leading Talents Project [CQYC20200303107, cstc2022ycjh-bgzxm0001]"

基金资助正文："The National Natural Science Foundation of China, 81873980, 81873972,the Science and Technology Project of Guizhou, Qiankehejichu-ZK [2022] 654, Science and Technology Fund of Guizhou Health Commission, gzwkj2022-175, the Chongqing Science Fund for Distinguished Young Scholars, cstc2019j-cyjjqX0028, Foundation for Innovative Research Groups of Chongqing Higher Education Institutions, CXQT20013,the Chongqing Talents-Innovation Leading Talents Project, CQYC20200303107, cstc2022ycjh-bgzxm0001."