Vitamin A-regulated ciliated cells promote airway epithelium repair in an asthma mouse model

作者全名："Tan, Wen; Lin, Jilei; Wang, Yaping; Yan, Li; Ying, Linyan; Dai, Jihong; Fu, Zhou; Liu, Jingyue"

作者地址："[Tan, Wen; Lin, Jilei; Wang, Yaping; Yan, Li; Ying, Linyan; Dai, Jihong; Fu, Zhou; Liu, Jingyue] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat,Childrens Hosp, Chongqing, Peoples R China; [Tan, Wen; Lin, Jilei; Yan, Li; Ying, Linyan; Dai, Jihong; Fu, Zhou; Liu, Jingyue] Chongqing Med Univ, Childrens Hosp, Dept Resp Med, Chongqing, Peoples R China; [Liu, Jingyue] Chongqing Med Univ, Childrens Hosp, Dept Resp Med, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China"

通信作者："Liu, JY (通讯作者)，Chongqing Med Univ, Childrens Hosp, Dept Resp Med, 136 Zhongshan 2nd Rd, Chongqing 400014, Peoples R China."

来源：ALLERGOLOGIA ET IMMUNOPATHOLOGIA

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000948868000001

JCR分区：Q3

影响因子：2.5

年份：2023

卷号：51

期号：1

开始页：116

结束页：125

文献类型：Article

关键词：airway epithelium; asthma; ciliated cell; vitamin A; treatment

摘要："Background: Asthma is a chronic inflammatory airway disease that causes damage to and exfoliation of the airway epithelium. The continuous damage to the airway epithelium in asthma cannot be repaired quickly and generates irreversible damage, repeated attacks, and aggravation. Vitamin A (VA) has multifarious biological functions that include maintaining membrane stability and integrity of the structure and function of epithelial cells. Our research explored the role of VA in repairing the airway epithelium and provided a novel treatment strategy for asthma. Methods: A mouse asthma model was established by house dust mite (HDM) and treated with VA by gavage. Human bronchial epithelial (16HBE) cells were treated with HDM and all-trans retinoic acid (ATRA) in vitro. We analyzed the mRNA and protein expression of characteristic markers, such as acetyl-alpha-tubulin (Ac-TUB) and FOXJ1 in ciliated cells and MUC5AC in secretory cells, mucus secretion, airway inflammation, the morphology of cilia, and the integrity of the airway epithelium. Results: Findings showed destruction of airway epithelial integrity, damaged cilia, high mucus secretion, increased MUC5AC expression, and decreased Ac-TUB and FOXJ1 expression in asthmatic mice. The VA intervention reversed the effect on Ac-TUB and FOXJ1 and promoted ciliated cells to repair the damage and maintain airway epithelial integrity. In 16HBE cells, we could confirm that ATRA promoted the expression of Ac-TUB and FOXJ1. Conclusion: These results demonstrated that VA-regulated ciliated cells to repair the damaged airway epithelium caused by asthma and maintain airway epithelial integrity. VA intervention is a potential adjunct to conventional treatment for asthma. (c) 2023 Codon Publications. Published by Codon Publications."

基金机构：National Natural Science Foundation of China [81900023]

基金资助正文：Acknowledgements This work was supported by the National Natural Science Foundation of China (No. 81900023) .