Enriching adenosine by thymine-rich DNA oligomers

作者全名："Liu, Mingchun; Chen, Huaiqing; Huang, Yuhan; Liu, Jian; Chen, Qianfeng; Zuo, Hua; Fang, Liang; Mao, Chengde"

作者地址："[Liu, Mingchun; Huang, Yuhan; Liu, Jian; Chen, Qianfeng; Zuo, Hua; Mao, Chengde] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China; [Chen, Huaiqing] Southwest Univ, Biol Sci Res Ctr, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China; [Fang, Liang] Ninth Chongqing Peoples Hosp, Dept Oncol, Chongqing 400700, Peoples R China; [Fang, Liang] Second Affiliated Hosp Chongqing Med Univ, Dept Hematol, Chongqing 400010, Peoples R China; [Mao, Chengde] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA"

通信作者："Mao, CD (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China.; Fang, L (通讯作者)，Ninth Chongqing Peoples Hosp, Dept Oncol, Chongqing 400700, Peoples R China.; Fang, L (通讯作者)，Second Affiliated Hosp Chongqing Med Univ, Dept Hematol, Chongqing 400010, Peoples R China.; Mao, CD (通讯作者)，Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA."

来源：ANALYST

ESI学科分类：CHEMISTRY

WOS号：WOS:000950819800001

JCR分区：Q2

影响因子：3.6

年份：2023

卷号：148

期号：8

开始页：1858

结束页：1866

文献类型：Article

关键词：　

摘要："Adenosine levels are important in various physiological and pathological activities, but detecting them is difficult because of interference from a complex matrix. This study designed a series of DNA oligomers rich in thymine to enrich adenosine. Their binding affinity (K-d range: 1.25-5.0 mM) to adenosine varied based on the DNA secondary structures, with a clamped hairpin structure showing the highest binding affinity. Compared to other designs, this clamped DNA hairpin underwent the least conformational change during adenosine binding. These DNAs also suppressed the precipitation of supersaturated adenine. Taken together, these results suggest that thymine-rich DNAs could be used to enrich and separate adenosine."

基金机构："National Science Foundation of China [21974111, 22274135]; Chongqing Research Program of Basic Research and Frontier Technology, China [cstc2021jcyj-msxmX0931, cstc2021jcyj-msxmX1033, cstc2020jcyj-msxmX0947]; Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China [2022NSFSC0682]"

基金资助正文："This work was supported by the National Science Foundation of China (21974111 and 22274135), the Chongqing Research Program of Basic Research and Frontier Technology, China (cstc2021jcyj-msxmX0931, cstc2021jcyj-msxmX1033, and cstc2020jcyj-msxmX0947), and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2022NSFSC0682)."