Dose-dependent effects of human umbilical cord-derived mesenchymal stem cell treatment in hyperoxia-induced lung injury of neonatal rats

作者全名："Xiong, Jing; Ai, Qing; Bao, Lei; Gan, Yuanshan; Dai, Xiaoyu; Han, Mei; Shi, Yuan"

作者地址："[Xiong, Jing; Ai, Qing; Bao, Lei; Shi, Yuan] Chongqing Med Univ, Childrens Hosp, Neonatal Diag & Treatment Ctr, Chongqing, Peoples R China; [Xiong, Jing; Ai, Qing; Bao, Lei; Shi, Yuan] Natl Clin Res Ctr Child Hlth & Disorders, Chongqing, Peoples R China; [Xiong, Jing; Ai, Qing; Bao, Lei; Shi, Yuan] Minist Educ, Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Xiong, Jing; Ai, Qing; Bao, Lei; Shi, Yuan] China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Xiong, Jing; Ai, Qing; Bao, Lei; Shi, Yuan] Chongqing Key Lab Pediat, Chongqing, Peoples R China; [Gan, Yuanshan; Dai, Xiaoyu; Han, Mei] Perfect Cell Biotechnol Co Ltd, Chongqing, Peoples R China"

通信作者："Shi, Y (通讯作者)，Chongqing Med Univ, Childrens Hosp, Neonatal Diag & Treatment Ctr, Chongqing, Peoples R China.; Shi, Y (通讯作者)，Natl Clin Res Ctr Child Hlth & Disorders, Chongqing, Peoples R China.; Shi, Y (通讯作者)，Minist Educ, Key Lab Child Dev & Disorders, Chongqing, Peoples R China.; Shi, Y (通讯作者)，China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China.; Shi, Y (通讯作者)，Chongqing Key Lab Pediat, Chongqing, Peoples R China."

来源：FRONTIERS IN PEDIATRICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000951071200001

JCR分区：Q2

影响因子：2.1

年份：2023

卷号：11

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：hyperoxia; human umbilical cord-derived mesenchymal stem cells; bronchopulmonary dysplasis; neonate; inflammation

摘要："BackgroundMesenchymal stem cells (MSCs) are multipotent stromal cells that have been reported to possess great potential for the treatment of bronchopulmonary dysplasia (BPD).ObjectiveOur study aims to assess the effects of three different doses of intraperitoneal administration of human umbilical cord-derived MSCs (hUC-MSCs) on a hyperoxia-induced BPD model of newborn rat.MethodsNeonatal Sprague Dawley (SD) rats were reared in either hyperoxia (75% O2) or room air (RA) from postnatal days (PN) 1-14. At PN5, hUC-MSCs (1 x 106, 5x 106,or 1x 107 cells per pup) were given intraperitoneally to newborn rats exposed to 75% O2 from birth; the controls received an equal volume of normal saline (NS). At PN14, the lung tissues, serum, and bronchoalveolar fluid (BALF) were collected for histologic examination, wet/dry (W/D) weight ratio analysis, engraftment, myeoloperoxidase (MPO) activity analysis, cytokine analysis, and western blot analysis of protein expression.ResultsCompared to rat pups reared in RA, rat pups reared in hyperoxia had a significant lower survival rate (53.3%) (P < 0.01). Hyperoxia-exposed rats exhibited pulmonary inflammation accompanied by alveolar-capillary leakage, neutrophile infiltration, augmented myeloperoxidase (MPO) activity, prominent alveolar simplification, and increased mean linear intercept (MLI), which was ameliorated by hUC-MSCs treatment. Increased oxidative stress and inflammatory cytokine production were also reduced. Importantly, the expression of Fas, an apoptosis-associated protein that was increasingly expressed in hyperoxia-exposed rats (P < 0.05), was downregulated after administration of hUC-MSCs (P < 0.05).ConclusionsOur results suggest that intraperitoneal administration of high number hUC-MSCs (1 x 107 cells) may represent an effective modality for the treatment of hyperoxia-induced BPD in neonatal rats."

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