Cryopreservation of human dental roots using vitrification for autologous human tooth tissue banking

作者全名："Wang, Jiang; Jiang, Peiru; Zheng, Chengxiang; Tang, Yin; Dou, Lei"

作者地址："[Wang, Jiang] Chongqing Med Univ, Women & Childrens Hosp, Chongqing, Peoples R China; [Jiang, Peiru; Zheng, Chengxiang; Dou, Lei] Chongqing Med Univ, Stomatol Hosp, 426 Song Shi Bei Rd, Chongqing 401147, Peoples R China; [Jiang, Peiru; Zheng, Chengxiang; Dou, Lei] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Jiang, Peiru; Zheng, Chengxiang; Dou, Lei] Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing, Peoples R China; [Tang, Yin] Univ Southern Calif, Herman Ostrow Sch Dent, Los Angeles, CA USA; [Tang, Yin] Bay Area Community Hlth Ctr, Fremont, CA USA"

通信作者："Dou, L (通讯作者)，Chongqing Med Univ, Stomatol Hosp, 426 Song Shi Bei Rd, Chongqing 401147, Peoples R China."

来源：CRYOBIOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000951071800011

JCR分区：Q2

影响因子：2.3

年份：2023

卷号：110

期号：　

开始页：86

结束页：92

文献类型：Article

关键词：Cryopreservation; Cell survival; Vitrification; Autologous; Tooth

摘要："This study aims to preliminarily evaluate the feasibility of autologous transplantation of tooth tissues cryopreserved with vitrification, by investigating the influence of cryopreservation with vitrification on human dental root, regarding the morphology, microhardness, cell apoptosis, proliferation and differentiation. Freshly extracted human permanent premolars were collected with crown removed. Dental roots were cryopreserved using a commercial vitrification medium (Kitazatousa). After six-month storage in liquid nitrogen, cryopreserved roots were thawed, and then evaluated using histological and immunohistochemical methods. Microhardness of dentine was measured with a Vickers indenter. Cells in periodontal ligament and dental pulp tissues were isolated and characterized. The proliferation, immunophenotype, apoptosis and differentiation ability of cells isolated from cryopreserved roots were evaluated. The data was analyzed using one-way analysis of variance (ANOVA) and Student's t-test. The gross and histological morphology of dental roots was not significantly changed after vitrification and thawing. A few tiny cracks were found in 3 of all 10 cryopreserved samples. No obvious changes were found in microstructure of dentine under SEM observation. Dental pulp cells and periodontal ligament cells were successfully isolated from tissues of cryopreserved human dental roots. There were also no significant differences of those periodontal ligament cells in the two groups regarding morphology, immunophenotype, viability, proliferation and apoptosis. The osteogenic and adipogenic differentiation capability of periodontal ligament cells was maintained by cryopreservation with vitrification. In the conditions of this study, cryopreservation with vitrification preserves cell survival, hardness and structural integrity of dental roots. Vitrification can be a potential way to preserve tooth tissue for future auto-transplantation and autologous cell therapy."

基金机构：National Natural Science Foundation of China [81800958]; Chongqing Young and Middle aged Medical High-end Talents Project

基金资助正文：This study was sponsored by National Natural Science Foundation of China (grant no. 81800958) and Chongqing Young and Middle aged Medical High-end Talents Project.