LncRNA HOXB-AS4 promotes proliferation and migration of colorectal cancer via the miR-140-5p/hdac7 axis

作者全名："Deng, Qican; Yang, Jianguo; Chen, Yajun; Chen, Zhenzhou; Li, Juncai; Fu, Zhongxue"

作者地址："[Deng, Qican; Yang, Jianguo; Li, Juncai; Fu, Zhongxue] Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing, Peoples R China; [Chen, Yajun; Chen, Zhenzhou] Chongqing Med Univ, Affiliated Hosp 3, Dept Gen Surg, Chongqing, Peoples R China"

通信作者："Fu, ZX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg, Chongqing, Peoples R China."

来源：BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000951844900001

JCR分区：Q1

影响因子：6.5

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：HOXB-AS4; colorectal cancer; MiR-140-5p; HDAC7

摘要："Long noncoding RNAs (lncRNA) have a critical role in colorectal cancer (CRC) development and progression. However, the role of the lncRNA HOXB-AS4 in CRC remains unclear. In this study, we found that HOXB-AS4 was markedly upregulated in tumor tissues compared to precancerous tissues. Loss-of-function assays in HT29 and SW480 cells confirmed that knockdown of HOXB-AS4 inhibited proliferation, migration, and promoted apoptosis. In addition, HOXB-AS4 was shown to regulate histone deacetylase 7 (HDAC7) expression by acting as a molecular sponge to bind to and adsorb miR-140-5p. These findings were confirmed by the dual-luciferase reporter assay. Functional recovery experiments further demonstrated the crucial role of the HOXB-AS4/miR-140-5p/HDAC7 axis in modulating the malignant phenotype of CRC cells. Collectively, our data suggested that HOXB-AS4 regulated the malignant tumor aggression of HT29 and SW480 cells through the miR-140-5p/HDAC7 axis and PI3K/AKT signaling pathway. Our study provides novel insights into the mechanism of action of HOXB-AS4 in CRC and highlights its potential use as a targeted therapy."

基金机构：Chongqing key disease Research and Application Demonstration Programm (Colorectal Cancer Prevention and Treatment Technology Research and Application Demonstration) [2019ZX003]

基金资助正文：This work was supported by Chongqing key disease Research and Application Demonstration Programm (Colorectal Cancer Prevention and Treatment Technology Research and Application Demonstration) (No.2019ZX003)