Changes of gut microbiota and tricarboxylic acid metabolites may be helpful in early diagnosis of necrotizing enterocolitis: A pilot study

作者全名："Du, Ting-Ting; Liu, Xiao-Chen; He, Yu; Gao, Xiong; Liu, Zhen-Zhen; Wang, Zheng-Li; Li, Lu-Quan"

作者地址："[Du, Ting-Ting; Liu, Xiao-Chen; He, Yu; Gao, Xiong; Liu, Zhen-Zhen; Wang, Zheng-Li; Li, Lu-Quan] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Neonatal Diag & Treatment Ctr,Chongqing Key Lab Pe, Chongqing, Peoples R China; [Li, Lu-Quan] Chongqing Med Univ, Jiangxi Hosp, Affiliated Childrens Hosp, Nanchang, Peoples R China"

通信作者："Li, LQ (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Neonatal Diag & Treatment Ctr,Chongqing Key Lab Pe, Chongqing, Peoples R China.; Li, LQ (通讯作者)，Chongqing Med Univ, Jiangxi Hosp, Affiliated Childrens Hosp, Nanchang, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000955008000001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：gut microbiota; succinate; tricarboxylic acid metabolites; NEC; early diagnosis

摘要："PurposeWe aimed to explore the value of gut microbiota and tricarboxylic acid (TCA) metabolites in early diagnosis of necrotizing enterocolitis (NEC) among infants with abdominal manifestations. MethodsThirty-two preterm infants with abdominal manifestations at gestational age <= 34 weeks were included in the study and were divided into non-NEC (n = 16) and NEC (n = 16) groups. Faecal samples were collected when the infants were enrolled. The gut microbiota was analysed with high-throughput sequencing, and TCA metabolites were measured with multiple reaction monitoring (MRM) targeted metabolomics. Receiver operating characteristic (ROC) curves were generated to explore the predictive value of the obtained data. ResultsThere was no significant difference in alpha diversity or beta diversity between the two groups (p > 0.05). At the phylum level, Proteobacteria increased, and Actinomycetota decreased in the NEC group (p < 0.05). At the genus level, Bifidobacterium and Lactobacillaceae decreased significantly, and at the species level, unclassified Staphylococcus, Lactobacillaceae and Bifidobacterium animalis subsp. lactis decreased in the NEC group (p < 0.05). Further Linear discriminant analysis effect sizes (LEfSe) analysis showed that the change in Proteobacteria at the phylum level and Lactobacillaceae and Bifidobacterium at the genus level scored higher than 4. The concentrations of succinate, L-malic acid and oxaloacetate in the NEC group significantly increased (p < 0.05), and the areas under the ROC curve for these metabolites were 0.6641, 0.7617, and 0.7344, respectively. ConclusionDecreased unclassified Staphylococcus, Lactobacillaceae and Bifidobacterium animalis subsp. lactis at the species level as well as the increase in the contents of some TCA metabolites, including succinate, L-malic acid and oxaloacetate, have potential value for the early diagnosis of NEC."

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