Comprehensive analysis of the FOXA1-related ceRNA network and identification of the MAGI2-AS3/DUSP2 axis as a prognostic biomarker in prostate cancer

作者全名："Yang, Guo; Chen, Xiong; Quan, Zhen; Liu, Miao; Guo, Yuan; Tang, Yangbin; Peng, Lang; Wang, Leilei; Wu, Yingying; Wu, Xiaohou; Liu, Jiayu; Zheng, Yongbo"

作者地址："[Yang, Guo; Quan, Zhen; Liu, Miao; Guo, Yuan; Tang, Yangbin; Peng, Lang; Wu, Xiaohou; Liu, Jiayu; Zheng, Yongbo] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, Chongqing, Peoples R China; [Chen, Xiong] Ninth Peoples Hosp Chongqing, Dept Urol, Chongqing, Peoples R China; [Wang, Leilei; Wu, Yingying] Chongqing Med Univ, Key Lab Lab Med Diagnost, Minist Educ, Chongqing, Peoples R China"

通信作者："Liu, JY; Zheng, YB (通讯作者)，Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：FRONTIERS IN ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000956948900001

JCR分区：Q2

影响因子：3.5

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：MAGI2-AS3; DUSP2; ceRNA; prostate cancer; FOXA1

摘要："Background: Prostate cancer (PCa) is the second most common cause of cancer-related deaths in American men. Even though increasing evidence has disclosed the competitive endogenous RNA (ceRNA) regulatory networks among cancers, the complexity and behavior characteristics of the ceRNA network in PCa remain unclear. Our study aimed to investigate the forkhead box A1 (FOXA1)-related ceRNA regulatory network and ascertain potential prognostic markers associated with PCa. Methods: RNA sequence profiles downloaded from The Cancer Genome Atlas (TCGA) were analyzed to recognize differentially expressed genes (DEGs) derived from tumor and non-tumor adjacent samples as well as FOXA1low and FOXA1high tumor samples. The enrichment analysis was conducted for the dysregulated mRNAs. The network for the differentially expressed long non-coding RNA (lncRNA)-associated ceRNAs was then established. Survival analysis and univariate Cox regression analysis were executed to determine independent prognostic RNAs associated with PCa. The correlation between DUSP2 and immune cell infiltration level was analyzed. Tissue and blood samples were collected to verify our network. Molecular experiments were performed to explore whether DUSP2 is involved in the development of PCa. Results: A ceRNA network related to FOXA1 was constructed and comprised 18 lncRNAs, 5 miRNAs, and 44 mRNAs. The MAGI2-AS3 similar to has-mir-106a/has-mir204 similar to DUSP2 ceRNA regulatory network relevant to the prognosis of PCa was obtained by analysis. We markedly distinguished the MAGI2-AS3/DUSP2 axis in the ceRNA. It will most likely become a clinical prognostic model and impact the changes in the tumor immune microenvironment of PCa. The abnormal MAGI2AS3 expression level from the patients' blood manifested that it would be a novel potential diagnostic biomarker for PCa. Moreover, down-expressed DUSP2 suppressed the proliferation and migration of PCa cells. Conclusions: Our findings provide pivotal clues to understanding the role of the FOXA1-concerned ceRNA network in PCa. Simultaneously, this MAGI2-AS3/ DUSP2 axis might be a new significant prognostic factor associated with the diagnosis and prognosis of PCa."

基金机构：National Natural Science Foundation of China (NSFC) [81802543]

基金资助正文：This study was supported by a grant from the projects of the National Natural Science Foundation of China (NSFC) (Grant No. 81802543).