Fusobacterium nucleatum promotes the early occurrence of esophageal cancer through upregulation of IL-32/PRTN3 expression

作者全名："Lei, Jing; Xu, Feng; Deng, Chao; Nie, Xubiao; Zhong, Li; Wu, Zhixuan; Li, Juan; Wu, Xiaoling; He, Song; Chen, Yongyu"

作者地址："[Lei, Jing; Xu, Feng; Deng, Chao; Nie, Xubiao; Zhong, Li; Wu, Zhixuan; Li, Juan; Wu, Xiaoling; He, Song; Chen, Yongyu] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, 74 Linjiang Rd, Chongqing 400010, Peoples R China"

通信作者："Wu, XL; He, S; Chen, YY (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, 74 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：CANCER SCIENCE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000958282800001

JCR分区：Q1

影响因子：4.5

年份：2023

卷号：　

期号：　

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结束页：　

文献类型：Article; Early Access

关键词：early-stage esophageal cancer; esophageal squamous cell carcinoma; Fn; IL-32; proliferation

摘要："Previous studies have shown that gastrointestinal microbiome is associated with the development of esophageal cancer, but the relationship and molecular mechanism between esophageal microbiota and the early development of esophageal cancer remain unclear. Here, we found that Lactobacillus, Escherichia-Shigella, Rikenellaceae-RC9-gut-group, Morganella, and Fusobacterium were more abundant in early-stage esophageal cancer (EEC) tissues compared with normal esophageal tissues. The abundance of bacteria such as Prevotella, Fusobacterium, Porphyromonas, Actinobacillus, and Neisseria in advanced esophageal cancer (AEC) was higher than that in EEC. Then, we further verified that Fusobacterium nucleatum (Fn) was enriched in EEC tissues and that its abundance increased with the progression of esophageal cancer by FISH and RT-PCR. Next, we demonstrated that Fn promoted the proliferation of esophageal squamous cell carcinoma (ESCC) in vitro and in vivo. Finally, we confirmed that Fn promoted ESCC proliferation by upregulating the expression of interleukin (IL)-32/proteinase 3 (PRTN3) and then activating the PI3K/AKT signaling pathway. In conclusion, Fn promoted the early development of ESCC by upregulating the expression of IL-32/PRTN3 and thereby activating the PI3K/AKT signaling pathway. A better understanding of the molecular mechanism of Fn in early esophageal cancer may contribute to the development of early screening markers to diagnose ESCC and provide new targets for treatment."

基金机构：National Natural Science Foundation of China [82100548]; Special support for Chongqing post doctoral research project [2010010005739004]

基金资助正文："National Natural Science Foundation of China, Grant/Award Number: 82100548; Special support for Chongqing post doctoral research project, Grant/Award Number: 2010010005739004"