The SDF-1/CXCR4 Signaling Pathway Directs the Migration of Systemically Transplanted Bone Marrow Mesenchymal Stem Cells Towards the Lesion Site in a Rat Model of Spinal Cord Injury

作者全名："Zhao, Andong; Chung, Manhon; Yang, Yi; Pan, Xiaohua; Pan, Yu; Cai, Sa"

作者地址："[Zhao, Andong; Yang, Yi; Pan, Xiaohua; Pan, Yu; Cai, Sa] Shenzhen Univ, Affiliated Hosp 2, Hlth Sci Ctr, Dept Trauma & Orthoped, Shenzhen, Peoples R China; [Zhao, Andong] Chongqing Med Univ, Affiliated Hosp 2, Plast & Maxillofacial Surg Dept, Chongqing, Peoples R China; [Chung, Manhon] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg, Sch Med, Shanghai, Peoples R China"

通信作者："Pan, Y; Cai, S (通讯作者)，Shenzhen Univ, Affiliated Hosp 2, Hlth Sci Ctr, Dept Trauma & Orthoped, Shenzhen, Peoples R China."

来源：CURRENT STEM CELL RESEARCH & THERAPY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000958781800006

JCR分区：Q4

影响因子：2.1

年份：2023

卷号：18

期号：2

开始页：216

结束页：230

文献类型：Article

关键词：Bone marrow mesenchymal stem cells; spinal cord injury; migration; SDF-1; CXCR4; neural regeneration

摘要："Background It has been observed that bone marrow-derived mesenchymal stem cells (MSCs) migrate towards the injured spinal cord and promote functional recovery when systemically transplanted into the traumatized spinal cord. However, the mechanisms underlying their migration to the spinal cord remain poorly understood. Methods In this study, we systemically transplanted GFP- and luciferase-expressing MSCs into rat models of spinal cord injury and examined the role of the stromal cell-derived factor 1 (SDF-1)/CXCR4 axis in regulating the migration of transplanted MSCs to the spinal cord. After intravenous injection, MSCs migrated to the injured spinal cord where the expression of SDF-1 was increased. Spinal cord recruitment of MSCs was blocked by pre-incubation with an inhibitor of CXCR4. Their presence correlated with morphological and functional recovery. In vitro, SDF-1 or cerebrospinal fluid (CSF) collected from SCI rats promoted a dose-dependent migration of MSCs in culture, which was blocked by an inhibitor of CXCR4 or SDF-1 antibody. Results and Conclusion The study suggests that SDF-1/CXCR4 interactions recruit exogenous MSCs to injured spinal cord tissues and may enhance neural regeneration. Modulation of the homing capacity may be instrumental in harnessing the therapeutic potential of MSCs."

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