Cryptotanshinone inhibits oral squamous cell carcinoma through the autophagic pathway

作者全名："Jiang, Xiao-Tian; Qiu, Ye; LI, Cong-Hua"

作者地址："[Jiang, Xiao-Tian] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Qiu, Ye] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [LI, Cong-Hua] Chongqing Med Univ, Stomatol Hosp, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Qiu, Y (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Li, CH (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：NEOPLASMA

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000961326500010

JCR分区：Q3

影响因子：2

年份：2023

卷号：70

期号：1

开始页：114

结束页：122

文献类型：Article

关键词：autophagy; cryptotanshinone; apoptosis; oral squamous cell carcinoma

摘要："Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors with a low quality of life. Because traditional surgical treatment often causes large wounds and then affects the quality of life of patients, it is urgent to find new and efficient drugs with good safety for clinical treatment. This study aimed to identify potential anticancer drugs starting from the traditional Chinese medicine Salvia miltiorrhiza extract. Cryptotanshinone, a compound isolated from the Chinese herb Salvia miltiorrhiza, was found to significantly induce apoptosis and inhibit proliferation in OSCC. By electron microscopy, autophagosomes were found. Confocal fluorescence microscopy data showed that cryptotanshinone signifi-cantly induced autophagy in OSCC cells. Mechanistically, the western blot assay indicated that cryptotanshinone induced cell autophagy through the activation of the LC3 pathway, whereas the autophagy inhibitor 3-methyladenine attenuated these effects. Furthermore, we demonstrated that cryptotanshinone had a significant antitumor effect in a tumor xenograft model, and no damage to vital organs was observed. Our findings provide evidence that cryptotanshinone may be a novel therapeutic strategy for the treatment of OSCC."

基金机构：Youth Fund of the National Natural Science Foundation of China [81901007]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [2021MSXM114]

基金资助正文：Acknowledgments: This work is supported by the Youth Fund of the National Natural Science Foundation of China (81901007) and the Scientific and Technological Research Program of Chongqing Municipal Education Commission (Grant No. 2021MSXM114) .