Roles of prokineticin 2 in electroconvulsive shock-induced memory impairment via regulation of phenotype polarization in astrocytes

作者全名："Chen, Lihao; Lv, Feng; Min, Su; Yang, You; Liu, Di"

作者地址："[Chen, Lihao; Lv, Feng; Min, Su; Yang, You; Liu, Di] Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Min, Su] Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Min, S (通讯作者)，Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Min, S (通讯作者)，Chongqing Med Univ, Dept Anesthesiol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：BEHAVIOURAL BRAIN RESEARCH

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000962211700001

JCR分区：Q2

影响因子：2.6

年份：2023

卷号：446

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Electroconvulsive shock; Phenotype polarization; Astrocytes; Prokineticin 2; Synaptic plasticity

摘要："Electroconvulsive shock (ECT) is the most effective treatment for depression but can impair learning and memory. ECT is increasingly being shown to activate astrocytes and induce neuroinflammation, resulting in cognitive decline. Activated astrocytes can differentiate into two subtypes, A1-type astrocytes and A2-type as-trocytes. Regarding cognitive function, neurotoxic A1 astrocytes and neuroprotective A2 astrocytes may exhibit opposite effects. Specifically, prokineticin 2 (PK2) functions as an essential mediator of inflammation and in-duces a selective A2-protective phenotype in astrocytes. This study aimed to clarify how PK2 promotes improved learning memory following electroconvulsive shock (ECS). As part of the study, rats were modeled using chronic unpredictable mild stress. Behavioral experiments were conducted to assess their cognitive abilities and depression-like behaviors. Western blot was used to determine the expression of PK2. Immunohistochemical and electron microscopy analyses of the hippocampal CA1 region were conducted to study the activation of astrocyte subtypes and synaptic ultrastructure, respectively. In this study, rats' spatial learning and memory impairment began to improve as activated A1-subtype astrocytes gradually decreased, and PK2 and A2 phenotype activation peaked on the third day after ECS. PKRA7 (PK2 antagonist) inhibits A2-type astrocyte activation partially and suppresses spatial learning and memory improvement. Collectively, our findings support that PK2 may induce a selective modulation of astrocytic polarization to a protective phenotype to promote learning and memory improvement after ECS"

基金机构：　

基金资助正文：