Landscape in the gallbladder mycobiome and bacteriome of patients undergoing cholelithiasis with chronic cholecystitis

作者全名："Hu, Junqing; Tang, Jichao; Zhang, Xinpeng; Yang, Kaijin; Zhong, Ayan; Yang, Qin; Liu, Yanjun; Li, Yi; Zhang, Tongtong"

作者地址："[Hu, Junqing; Tang, Jichao; Zhang, Xinpeng; Yang, Kaijin; Zhong, Ayan; Yang, Qin; Liu, Yanjun; Li, Yi; Zhang, Tongtong] Chongqing Med Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2, Affiliated Hosp,Southwest Jiaotong Univ,Ctr Gastro, Chengdu, Peoples R China; [Hu, Junqing; Tang, Jichao; Zhang, Xinpeng; Yang, Kaijin; Zhong, Ayan; Yang, Qin; Liu, Yanjun; Li, Yi; Zhang, Tongtong] Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Ctr Obesi & Metab Hlth,A, Chengdu, Peoples R China; [Hu, Junqing; Zhang, Tongtong] Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Med Res Ctr,Affiliated H, Chengdu, Peoples R China; [Tang, Jichao; Zhang, Xinpeng; Yang, Kaijin; Zhong, Ayan; Li, Yi] Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Affiliated Hosp,Dept Ge, Chengdu, Peoples R China; [Yang, Qin] Affiliated Hosp Southwest Jiaotong Univ, Chongqing Med Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Sect Hepatopancreatobili, Chengdu, Peoples R China"

通信作者："Liu, YJ; Li, Y; Zhang, TT (通讯作者)，Chongqing Med Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2, Affiliated Hosp,Southwest Jiaotong Univ,Ctr Gastro, Chengdu, Peoples R China.; Liu, YJ; Li, Y; Zhang, TT (通讯作者)，Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Ctr Obesi & Metab Hlth,A, Chengdu, Peoples R China.; Zhang, TT (通讯作者)，Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Med Res Ctr,Affiliated H, Chengdu, Peoples R China.; Li, Y (通讯作者)，Chongqing Med Univ, Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Affiliated Hosp Chengdu 2,Affiliated Hosp,Dept Ge, Chengdu, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000963246300001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：gallstone disease; marker gene sequencing; microbiota; bile and gallstone; bacteria; fungi

摘要："Gallstone disease (GSD) is associated with changes in the gut and gallbladder bacterial composition, but there is limited information on the role of the fungal community (mycobiome) in disease development. This study aimed to characterize the gallbladder mycobiome profiles and their interactions with bacteriome in GSD. A total of 136 bile and gallstone samples (34 paired for bacteriome, and 33 paired and extra 2 bile samples for mycobiome) were obtained from calculi patients with chronic cholecystitis. Bile and gallstone bacteriome and mycobiome were profiled by 16S and internal transcribed spacer (ITS) rRNA gene sequencing, respectively. Gallbladder bacteriome, mycobiome, and interkingdom and intrakingdom interactions were compared between bile and gallstone. In general, microbial diversity was higher in bile than in gallstone, and distinct microbial community structures were observed among them. Deep Sea Euryarchaeotic Group, Rhodobacteraceae, and Rhodobacterales were microbial biomarkers of bile, while Clostridiales and Eubacterium coprostanoligenes were biomarkers of gallstone. Five fungal taxa, including Colletotrichum, Colletotrichum sublineola, and Epicoccum, were enriched in gallstone. Further ecologic analyses revealed that intensive transkingdom correlations between fungi and bacteria and intrakingdom correlations within them observed in gallstone were significantly decreased in bile. Large and complex fungal communities inhabit the gallbladder of patients with GSD. Gallstone, compared with bile, is characterized by significantly altered bacterial taxonomic composition and strengthened bacterial-bacterial, fungal-fungal, and bacterial-fungal correlations in the gallbladder of patients with GSD."

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