The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells

作者全名："Deng, Pingmeng; Huang, Jing; Zhang, Qixuan; Li, Yuejia; Li, Jie"

作者地址："[Deng, Pingmeng; Huang, Jing; Zhang, Qixuan; Li, Yuejia; Li, Jie] Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China; [Deng, Pingmeng; Huang, Jing; Zhang, Qixuan; Li, Yuejia; Li, Jie] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Deng, Pingmeng; Huang, Jing; Zhang, Qixuan; Li, Yuejia; Li, Jie] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Li, J (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshibei Rd, Chongqing 401147, Peoples R China.; Li, J (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Li, J (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：BMC ORAL HEALTH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000963457600003

JCR分区：Q1

影响因子：2.6

年份：2023

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Elastin microfibril interface-located protein-1; Human dental pulp stem cells; Osteo; Odontogenic differentiation; Tooth regeneration; Bone regeneration

摘要："BackgroundHuman dental pulp stem cells (hDPSCs) may be the best choice for self-repair and regeneration of teeth and maxillofacial bone tissue due to their homogeneous tissue origin, high proliferation and differentiation rates, and no obvious ethical restrictions. Recently, several studies have shown that extracellular matrix (ECM) proteins can effectively regulate the proliferation and differentiation fate of mesenchymal stem cells (MSCs). However, the role of elastin microfibril interface-located protein-1 (EMILIN-1), a new ECM glycoprotein, in osteo/odontogenic differentiation of hDPSCs has not been reported. The aim of this study was to explore the effect of EMILIN-1 during osteo/odontogenic differentiation of hDPSCs. MethodshDPSCs were cultured in osteo/odontogenic induction medium. qPCR and Western blot analysis were performed to detect osteo/odonto-specific genes/proteins expression as well as the expression of EMILIN-1. After knockdown of Emilin-1 in hDPSCs with small interfering RNA and exogenous addition of recombinant human EMILIN-1 protein (rhEMILIN-1), Cell Counting Kit-8 assay, alkaline phosphatase staining, alizarin red S staining, qPCR and Western blot were performed to examine the effect of EMILIN-1 on proliferation and osteo/odontogenic differentiation of hDPSCs. ResultsDuring the osteo/odontogenic induction of hDPSCs, the expression of osteo/odonto-specific genes/proteins increased, as did EMILIN-1 protein levels. More notably, knockdown of Emilin-1 decreased hDPSCs proliferation and osteo/odontogenic differentiation, whereas exogenous addition of rhEMILIN-1 increased them. ConclusionsThese findings suggested that EMILIN-1 is essential for the osteo/odontogenic differentiation of hDPSCs, which may provide new insights for teeth and bone tissue regeneration."

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