Recovery of host adaptive immune function promoted the reduction of hepatitis B surface antigen in nucleoside analog-experienced chronic hepatitis B patients with low hepatitis B surface antigen levels

作者全名："Zhang, Qian; Liu, Xiaoqing; Pang, Xicheng; Wang, Huimin; Du, Jinjie; Ren, Hong; Hu, Peng"

作者地址："[Zhang, Qian] Guizhou Prov Peoples Hosp, Dept Infect Dis, Guiyang 550002, Guizhou, Peoples R China; [Zhang, Qian; Liu, Xiaoqing; Pang, Xicheng; Wang, Huimin; Du, Jinjie; Ren, Hong; Hu, Peng] Chongqing Med Univ, Inst Viral Hepatitis, Dept Infect Dis, Key Lab Mol Biol Infect Dis,Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Hu, P (通讯作者)，Chongqing Med Univ, Inst Viral Hepatitis, Dept Infect Dis, Key Lab Mol Biol Infect Dis,Affiliated Hosp 2, Chongqing, Peoples R China."

来源：CYTOKINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000963944400001

JCR分区：Q2

影响因子：3.7

年份：2023

卷号：164

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Hepatitis B; Nucleoside analog therapy; Hepatitis B s antigen; Cytokines; Adaptive immune function

摘要："Hepatitis B surface antigen (HBsAg) seroclearance is an optimal therapeutic endpoint, as it reflects the amount of covalently closed circular DNA. The exact mechanisms that contribute to HBsAg reduction are not completely understood. We evaluated adaptive immunity in nucleoside analog-experienced chronic hepatitis B (CHB) pa-tients with low HBsAg levels who received oral antiviral therapy. One hundred and ninety-five CHB patients had hepatitis B virus (HBV) DNA <= 1000 IU/ml and HBsAg < 3000 IU/ml for longer than one year of antiviral therapy. According to HBsAg levels, they were divided into Group 1 (HBsAg reduction >= 0.5 log10) and Group 2 (HBsAg reduction < 0.5 log10). Cytokines, adaptive immune cells, and molecular markers in peripheral blood were detected at follow-up times. In total, 38 (19.5%) of the 195 patients achieved HBsAg reduction >= 0.5 log10. IL4, IL5, IL10, TGF beta, IL17, and PD-1 decreased gradually in these patients. HBsAg reduction had a link to the change in ICOSL+CD19+ B cells and CD40L+CXCR5+CD4+ Tfh cells. More CD8+ naive T lymphocytes differ-entiated into CD4+ TCMs, CD8+ TCMs and CD8+ TEMs in Group 1. Meanwhile, Group 1 exhibited elevated Th1 and Th1/Th2 levels and reduced levels of Treg versus those in Group 2. With the reduction in HBsAg, the imbalance of T-cell subsets was partially corrected; the immune activity of T cells was enhanced, and the state of immune exhaustion was alleviated to a certain extent."

基金机构："National Science and Technology Major Project of China [2017ZX10202203-007, 2017ZX10202203-008, 2018ZX10302-206-003]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University; Second Affiliated Hospital of Chongqing Medical University N/A; Science and Technology Fund Project of Guizhou Provincial Health Commission [gzwjkj2020-1-043]"

基金资助正文："This work was supported by the National Science and Technology Major Project of China [grant numbers 2017ZX10202203-007, 2017ZX10202203-008, and 2018ZX10302-206-003] , the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University N/A, Remarkable Innovation-Clinical Research Project, The Second Affiliated Hospital of Chongqing Medical University N/A, and the Science and Technology Fund Project of Guizhou Provincial Health Commission gzwjkj2020-1-043."