Requiring Reconsideration of Differences of Aeromonas Infections Between Extra-Intestinal and Intestinal in Hospitalized Patients

作者全名："Zhang, Daiqin; Li, Wenting; Hu, Xin; Huang, Hongyu; Zhang, Xiaobing"

作者地址："[Zhang, Daiqin; Li, Wenting; Hu, Xin; Huang, Hongyu; Zhang, Xiaobing] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, Chongqing, Peoples R China; [Zhang, Xiaobing] Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Zhang, XB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Lab Med, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：INFECTION AND DRUG RESISTANCE

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000964194200001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：16

期号：　

开始页：487

结束页：497

文献类型：Article

关键词：intestinal and extra-intestinal infections; Aeromonas; risk factor; virulence

摘要："Purpose: The purpose of this study is to examine the variations between extra-intestinal and intestinal infections of Aeromonas in terms of strain types, risk factors, drug susceptibility results, and the distribution of drug resistance and virulence genes. Patients and Methods: A total of 188 Aeromonas strains were identified to the species level using housekeeping genes (rpoD, gyrB, and gyrA). The risk factors for Aeromonas extra-intestinal and intestinal infection, as well as mortality, were retrospectively examined in this study. The broth microdilution method was used to investigate the antimicrobial susceptibility profiles. Touchdown polymerase chain reaction (PCR) assays and DNA sequencing were employed to confirm virulence and the presence of drug resistance genes.Results: The housekeeping genes identified 188 strains into 7 species. Extra-intestinal isolates generally contained A. caviae and A. hydrophila, while intestinal were A. veronii (p=0.0001). Extra-intestinal infections (158/188) were the main type and accounted for 24/27 of all fatalities. Malignant tumors, hepatobiliary diseases, anemia, and hypoproteinemia were linked to infections. Poor results were associated with septic shock. Using the broth microdilution method, over 80% isolates were susceptible to most antimicrobials, except for ceftazidime (79.8%) and ceftriaxone (69.7%). Except for imipenem, intestinal strains were more susceptible to other medications than extra-intestinal. Using touch-down polymerase chain reaction testing and DNA sequencing, 6 strains, 31 strains, and a strain only had blaTEM, blaCphA, and blaVIM, respectively. Two Aeromonas hydrophila each possessed blaCphA+ blaCTXM-M-9, and blaCphA + blaCTX-M-1 + blaCTX-M-15-like + blaTEM; two Aeromonas caviae each possessed blaNDM + blaCTX-M-1 +blaCTX-M-15-like + blaTEM, and blaNDM + blaTEM. Thirty-four of the 42 strains mentioned above were isolated from extra-intestinal. Act, aexT, and ascF-G, were in intestinal more frequently, but alt, hlyA, ela, and lip were in extra-intestinal more frequently. Conclusion: Aeromonas inside and outside intestinal differed in their clinical characteristics, drug susceptibility, drug resistance and virulence genes."

基金机构：Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0253]

基金资助正文："Funding This work was supported by the Natural Science Foundation of Chongqing (No. cstc2019jcyj-msxmX0253) . The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript."