Low-Intensity Pulsed Ultrasound Attenuates Periodontal Ligament Cells Apoptosis by Activating Yes-Associated Protein-Regulated Autophagy

作者全名："Jian, Zixiang; Li, Yao; Zhang, Chuangwei; Zhong, Wenjie; Ai, Dongqing; He, Yao; Song, Jinlin"

作者地址："[Jian, Zixiang; Zhang, Chuangwei; Zhong, Wenjie; Ai, Dongqing; He, Yao; Song, Jinlin] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Jian, Zixiang; Zhang, Chuangwei; Zhong, Wenjie; Ai, Dongqing; He, Yao; Song, Jinlin] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Jian, Zixiang; Zhang, Chuangwei; Zhong, Wenjie; Ai, Dongqing; He, Yao; Song, Jinlin] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Li, Yao] Peking Univ Sch & Hosp Stomatol, NMPA Key Lab Dent Mat, Natl Engn Lab Digital & Mat Technol Stomatol, Dept Geriatr Dent, Beijing, Peoples R China; [He, Yao; Song, Jinlin] Chongqing Med Univ, Dept Orthodont, Stomatol Hosp, 426 North Songshi Ave, Chongqing 401147, Peoples R China"

通信作者："He, Y; Song, JL (通讯作者)，Chongqing Med Univ, Dept Orthodont, Stomatol Hosp, 426 North Songshi Ave, Chongqing 401147, Peoples R China."

来源：ULTRASOUND IN MEDICINE AND BIOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000964966800001

JCR分区：Q2

影响因子：2.4

年份：2023

卷号：49

期号：5

开始页：1227

结束页：1237

文献类型：Article

关键词：Periodontitis; Low-intensity pulsed ultrasound; Periodontal ligament cells; Apoptosis; Yes-associated protein; Autophagy; Lipopolysaccharide; Alveolar bone; Rat; Small interfering RNA transfection

摘要："Objective: The goal of the work described here was to determine if low-intensity pulsed ultrasound (LIPUS) has an anti-inflammatory effect on lipopolysaccharide (LPS)-induced inflammation in periodontal ligament cells (PDLCs). The mechanism underlying this effect remains to be explored and is likely related to PDLC apoptosis reg-ulated by Yes-associated protein (YAP) and autophagy.Methods: To verify this hypothesis, we used a rat model of periodontitis and primary human PDLCs. We examined alveolar bone resorption in rats and apoptosis, autophagy and YAP activity in LPS-treated PDLCs with and without application of LIPUS by cellular immunofluorescence, transmission electron microscopy and Western blotting. Then, siRNA transfection was used to decrease YAP expression to confirm the regulatory role of YAP in the anti-apoptotic effect of LIPUS on PDLCs.Discussion: We found that LIPUS attenuated alveolar bone resorption in rats and this was accompanied by YAP activation. LIPUS inhibited hPDLC apoptosis by YAP activation, and promoted autophagic degradation to help autophagy completion. These effects were reversed after YAP expression was blocked.Conclusion: LIPUS attenuates PDLC apoptosis by activating Yes-associated protein-regulated autophagy."

基金机构：Natural Science Foundation of China [81771082]; Postdoctoral Research Foundation of China [2018M640902]; Postdoc-toral Program of Chongqing [XmT2018079]; Chongqing Graduate Tutor Team [dstd201903]

基金资助正文：Acknowledgments This work was supported by the Natural Science Foundation of China (Grant 81771082) and Postdoctoral Research Foundation of China (Grant 2018M640902) . Special support was received for the Postdoc-toral Program of Chongqing (Grant XmT2018079) and the Chongqing Graduate Tutor Team (Grant dstd201903) .