Ganoderma lucidum polysaccharides ameliorates D-galactose-induced aging salivary secretion disorders by upregulating the rhythm and aquaporins

作者全名："Wu, Mengna; Huang, Boyue; Hu, Ling; Zhang, Tao; Zhang, Binyu; Zhao, Xi; Lu, Ruijin; Xiong, Wei; Zhang, Shengyao; Li, Jing; Chen, Dilong; Yang, Baoxue; Li, Guoli; Ran, Jianhua"

作者地址："[Zhang, Binyu; Zhao, Xi; Zhang, Shengyao; Ran, Jianhua] Chongqing Med Univ, Basic Med Coll, Dept Anat, Lab Neurosci & Tissue Engn, Chongqing 400016, Peoples R China; [Lu, Ruijin; Xiong, Wei; Li, Jing; Chen, Dilong; Li, Guoli] Chongqing Med Univ, Basic Med Coll, Dept Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China; [Xiong, Wei; Chen, Dilong; Li, Guoli] Chongqing Three Gorges Med Coll, Dept Anat, Chongqing 404120, Peoples R China; [Xiong, Wei; Chen, Dilong; Li, Guoli; Ran, Jianhua] Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, Chongqing 404100, Peoples R China; [Yang, Baoxue] Peking Univ, Sch Basic Med Sci, Dept Pharmacol, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China"

通信作者："Ran, JH (通讯作者)，Chongqing Med Univ, Basic Med Coll, Dept Anat, Lab Neurosci & Tissue Engn, Chongqing 400016, Peoples R China.; Li, GL (通讯作者)，Chongqing Med Univ, Basic Med Coll, Dept Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China."

来源：EXPERIMENTAL GERONTOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000965564600001

JCR分区：Q2

影响因子：3.3

年份：2023

卷号：175

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Ganoderma lucidum polysaccharide; Aging; Submandibular gland; Aquaporins; Rhythm

摘要："Longer-term deterioration in saliva secretion has been observed to occur in response to aging. The functional deterioration of the salivary gland damages swallowing and chewing abilities and consequently reduces life quality of the elderly. There are, however, only a few proven effective treatments for aging salivary secretion disorders. Ganoderma lucidum polysaccharide (GLP) has been applied to treat various diseases because of its safety, efficacy, and low cost. We investigated the protective effect of GLP on the submandibular gland (SMG) during aging. D-galactose (D-gal) was used to treat the aging mice, and the body weight, water consumption, saliva secretion, and flow rate were measured after 6 weeks of modeling. Micromorphological changes of the SMG were assessed by hematoxylin-eosin staining and transmission electron microscopy. RT-qPCR and Western blot were used to detect the expression of apoptotic proteins and inflammatory cytokines. Aquaporins (AQPs) and rhythmic protein expression were analyzed by immunohistochemistry and immunofluorescence. The results showed that GLP effectively promoted the expression of AQP5, AQP4, and AQP1, inhibited the release of TNF-alpha, IL-6, and Bax, and reduced inflammation and apoptosis. Further experiments showed that GLP promoted the up-regulation of core clock genes and proteins and restored the co-localized expression of CLOCK and AQP5 that were weakened during aging, helping to attenuate aging-induced weight loss, decreased salivation, and struc-tural and functional damage. The findings of this work contribute to understanding the nature of age-related modifications in SMG by identifying changes in AQP5 expression and regulatory mechanisms linked to SMG dysfunction during aging. GLP is a potential drug for maintaining healthy salivary gland (SG) status and pre -venting SG deficiency in the elderly."

基金机构："National Natural Science Foundation of China [81770738]; Scientific Technology Project of Chongqing State Education Commission [KJQN202002710]; Chongqing Key Laboratory Project of Exploitation and Utilization of Crude Medicinal Materials in the Three Gorges Reservoir Area [KFKT202201, KFKT2022010]"

基金资助正文："This study was financially supported by the National Natural Science Foundation of China (81770738) , the Scientific Technology Project of Chongqing State Education Commission (KJQN202002710) , and the Chongqing Key Laboratory Project of Exploitation and Utilization of Crude Medicinal Materials in the Three Gorges Reservoir Area (KFKT202201, KFKT2022010) ."