Current understanding of gliomagenesis: from model to mechanism

作者全名："Luo, Juanjuan; Junaid, Muhammad; Hamid, Naima; Duan, Jin-Jing; Yang, Xiaojun; Pei, De-Sheng"

作者地址："[Luo, Juanjuan; Junaid, Muhammad; Hamid, Naima; Duan, Jin-Jing; Pei, De-Sheng] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 41016, Peoples R China; [Luo, Juanjuan; Yang, Xiaojun] Shantou Univ, Med Coll, Guangdong Prov Key Lab Infect Dis & Mol Immunopat, Shantou 515041, Peoples R China; [Junaid, Muhammad; Hamid, Naima] Univ Chinese Acad Sci, Beijing 100049, Peoples R China"

通信作者："Pei, DS (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 41016, Peoples R China.; Yang, XJ (通讯作者)，Shantou Univ, Med Coll, Guangdong Prov Key Lab Infect Dis & Mol Immunopat, Shantou 515041, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MEDICAL SCIENCES

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000965672100008

JCR分区：Q2

影响因子：3.6

年份：2022

卷号：19

期号：14

开始页：2071

结束页：2079

文献类型：Review

关键词：Glioma; Tumorigenesis; Signaling pathways; CRISPR/Cas9; Model

摘要："Glioma, a kind of central nervous system (CNS) tumor, is hard to cure and accounts for 32% of all CNS tumors. Establishing a stable glioma model is critically important to investigate the underlying molecular mechanisms involved in tumorigenesis and tumor progression. Various core signaling pathways have been identified in gliomagenesis, such as RTK/RAS/PI3K, TP53, and RB1. Traditional methods of establishing glioma animal models have included chemical induction, xenotransplantation, and genetic modifications (RCAS/t- va system, Cre-loxP, and TALENs). Recently, CRISPR/Cas9 has emerged as an efficient gene editing tool with high germline transmission and has extended the scope of stable and efficient glioma models that can be generated. Therefore, this review will highlight the documented evidence about the molecular characteristics, critical genetic markers, and signaling pathways responsible for gliomagenesis and progression. Moreover, methods of establishing glioma models using gene editing techniques and therapeutic aspects will be discussed. Finally, the prospect of applying gene editing in glioma by using CRISPR/Cas9 strategy and future research directions to establish a stable glioma model are also included in this review. In-depth knowledge of glioma signaling pathways and use of CRISPR/Cas9 can greatly assist in the development of a stable, efficient, and spontaneous glioma model, which can ultimately improve the effectiveness of therapeutic responses and cure glioma patients."

基金机构："High-level Talents Project of Chongqing Medical University [R4014]; CAS Team Project of the Belt and Road, Research Program of Chongqing Science and Technology Commission [cstc2019jcyj-zdxmX0035, CSTCCXLJRC201714]"

基金资助正文："This work was supports by grants from the High-level Talents Project of Chongqing Medical University (R4014), CAS Team Project of the Belt and Road, Research Program of Chongqing Science and Technology Commission (cstc2019jcyj-zdxmX0035, CSTCCXLJRC201714)."