Coumarin-based fluorescence turn-on probes for high selectivity peroxynitrite detection and imaging in living cells and gamma-carrageenan-induced inflammatory tissue and mice

作者全名：Wei Xiao-Ping; Li Rong; Zhu Min; Zhao Lulu; Ran Hongyan; Pang Meiling; Zhu Gao-Hui

作者地址："[Wei Xiao-Ping] Chongqing Med Univ, Childrens Hosp, Dept Pediat Res Inst,Chongqing Key Lab Child Nutr, Natl Clin Res Ctr Child Hlth & Disorders,Minist E, Chongqing 400014, Peoples R China; [Li Rong; Zhu Min; Zhu Gao-Hui] Chongqing Med Univ, Childrens Hosp, Dept Endocrinol,Chongqing Key Lab Pediat, Natl Clin Res Ctr Child Hlth & Disorders,Minist E, Chongqing 400014, Peoples R China; [Zhao Lulu; Ran Hongyan; Pang Meiling] Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Pharmaceut Metab Res, Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China"

通信作者："Zhu, GH (通讯作者)，Chongqing Med Univ, Childrens Hosp, Dept Endocrinol,Chongqing Key Lab Pediat, Natl Clin Res Ctr Child Hlth & Disorders,Minist E, Chongqing 400014, Peoples R China."

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000965726700005

JCR分区：Q1

影响因子：4.8

年份：2022

卷号：183

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Peroxynitrite anion (ONOO-); Reactive nitrogen species (RNS); Inflammatory tissues; Fluorescent probe

摘要："Peroxynitrite anion (ONOO-), a type of reactive nitrogen species (RNS), profoundly implicated in the physiological and pathological processes of a series of oxidative stress-related diseases. Thus, construction of reliable tools to track peroxynitrite (ONOO-) in biosystems is urgently desired. In this work, based-on D-pi-D-structure of coumarin derivative and 2-(4-nitrophenyl)-2-oxoacetamide (also served as the fluorescence quencher) as flourophore and response group, respectively, two fluorescence turn-on probes CMONOO1 and CMONOO2 were constructed for rapidly detection and imaging of ONOO- in living cells, and inflammatory tissues and mice. These two probes had fast response time for ONOO- (similar to 60 s), and exhibited highly selectivity and sensitivity detection performance for ONOO- over other ROS and RNS. Also they had excellent detection limits in the ranges of 0-1.0 mu M as low as 23.3 nM and 21.4 nM respectively. In addition, both probes have markedly biocompatibility, which could be beneficial to the imaging analysis of ONOO- in living biosystems. Therefore, CMONOO2 was selected as a representative for ONOO- imaging analysis in living cells, and inflammatory tissues and mice with satisfactory results, demonstrating these two robust probes have great potential to further elucidate ONOO--related diseases."

基金机构：national key research and development programme of China [2021YFC2701900]; National Natural Science Foundation of China [81602844]

基金资助正文："This work was supported by the national key research and development programme of China (2021YFC2701900), and National Natural Science Foundation of China (81602844)."