A novel electrochemical immunosensor based on rifter-like Ni-TCPP(Fe) nanosheets and PSS-functionalized graphene for ultrasensitive detection of H-FABP

作者全名："Zhang, Zixuan; Liu, Jie; Li, Ying; Dong, Jie; Qiu, Jinfu; Li, Chaorui"

作者地址："[Zhang, Zixuan; Liu, Jie; Li, Ying; Dong, Jie; Qiu, Jinfu; Li, Chaorui] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China"

通信作者："Li, CR (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China."

来源：JOURNAL OF SOLID STATE ELECTROCHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000966176800001

JCR分区：Q3

影响因子：2.6

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Ni-TCPP(Fe); PSS-Gr; Ag@Au; Pt; h-FABP; Electrochemical immunosensor

摘要："In terms of medicine, the prevention, diagnosis, and assessment of acute myocardial infarction (AMI) as well as the effectiveness of therapy all depend on the sensitive and early detection of human heart-type fatty acid-binding protein (H-HABP). The synthetic material used in this study, Ni-TCPP(Fe), has a unique structure that resembles a rifter with many thorns. When exposed to PSS-Gr, Ni-TCPP(Fe) would unfold into a sheet structure, exposing a lot of active sites and improving the catalytic active surface area, making it easier to detect H-FABP. We have successfully synthesized an electrochemical immunosensor based on Ni-TCPP(Fe)@PSS-Gr and Ag@Au/Pt as a signal label which demonstrated excellent sensor performance. A broad linear range of 10 fg/mL to 100 ng/mL with a low detection limit of 5.75 fg/mL (S/N = 5) for detecting H-FABP was obtained under ideal conditions. Furthermore, the detection results from the human serum samples were positive, suggesting that the made-up immunosensor could be useful for early clinical analysis."

基金机构："Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0121]"

基金资助正文："This work was supported by the Natural Science Foundation of Chongqing, China (No. cstc2019jcyj-msxmX0121). The ethical review number for human serum is 2020-542."