Dauer larva-derived extracellular vesicles extend the life of Caenorhabditis elegans

作者全名："Ma, Jing; Wang, Yi-ting; Chen, Ling-hui; Yang, Bang-ya; Jiang, Yong-zhu; Wang, Lan-xi; Chen, Zhi-qi; Ma, Guan-rong; Fang, Liao-qiong; Wang, Zhi-biao"

作者地址："[Ma, Jing; Wang, Yi-ting; Chen, Ling-hui; Yang, Bang-ya; Jiang, Yong-zhu; Wang, Lan-xi; Chen, Zhi-qi; Ma, Guan-rong; Fang, Liao-qiong; Wang, Zhi-biao] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Fang, Liao-qiong; Wang, Zhi-biao] Natl Engn Res Ctr Ultrasound Med, Chongqing 401121, Peoples R China"

通信作者："Fang, LQ; Wang, ZB (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China."

来源：BIOGERONTOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000967798600001

JCR分区：Q1

影响因子：4.4

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：C; elegans; Dauer; Extracellular vesicles (EVs); Lifespan

摘要："There is growing evidence that extracellular vesicles (EVs) play a functional role in tissue repair and anti-aging by transferring the contents of donor cells to recipient cells. We hypothesized that Dauer (C. elegans), known as ""ageless"" nematodes, can also secrete extracellular vesicles and influence the lifespan of C. elegans. Here, we isolated EVs of dauer larvae (dauer EVs). Dauer EVs were characterized using transmission electron microscopy, nanoparticle tracking analysis (NTA), and Western blot analysis. Wild-type C. elegans were fed in the presence or absence of dauer EVs and tested for a range of phenotypes, including longevity, mobility and reproductive capacity. Results showed that dauer EVs increased the average lifespan of nematodes by 15.74%, improved mobility, slowed age-related pigmentation as well as body length, and reduced the accumulation of reactive oxygen species and lipids, while not impairing nematode reproductive capacity. These findings suggest that dauer EVs can extend the lifespan of C. elegans as well as the healthy lifespan by reducing ROS accumulation, with potential anti-aging capacity."

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