"miR-526b-5p/c-Myc/Foxp1 participates in recurrent spontaneous abortion by regulating the proliferation, migration, and invasion of trophoblasts"

作者全名："Luo, Li; Yao, Lu; Xie, Youlong; Chen, Enxiang; Ding, Yubin; Ge, Luxing"

作者地址："[Luo, Li; Yao, Lu; Xie, Youlong; Ge, Luxing] Chongqing Med Univ, Sch Publ Hlth, Joint Int Res Lab Reprod & Dev, Minist Educ China, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Luo, Li; Yao, Lu; Ding, Yubin] Chongqing Med Univ, Dept Obstet & Gynecol, Women & Childrens Hosp, 120,Longshan Rd, Chongqing 401147, Peoples R China; [Chen, Enxiang] Chongqing Med Univ, Sch Basic Med, Dept Genet, Chongqing 400016, Peoples R China"

通信作者："Ge, LX (通讯作者)，Chongqing Med Univ, Sch Publ Hlth, Joint Int Res Lab Reprod & Dev, Minist Educ China, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China.; Ding, YB (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Women & Childrens Hosp, 120,Longshan Rd, Chongqing 401147, Peoples R China."

来源：JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000968301300001

JCR分区：Q1

影响因子：3.2

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：miR-526b-5p; c-Myc; Foxp1; Trophoblast; RSA

摘要："PurposeAs a member of the C19MC family, miR-526b-5p is mainly expressed in the placental tissue and is a well-known tumor suppressor microRNA. However, its effect on the function of trophoblasts and its role in the development of recurrent spontaneous abortion (RSA) remains unclear.MethodsTranscriptome sequencing, quantitative real-time polymerase chain reaction (RT-qPCR), Western blot, 5-ethynyl-2 '-deoxyuridine (Edu) proliferation analysis, cell counting kit-8 (CCK8) assay, Transwell assays, and wound healing were used to detect the proliferation, migration, and invasion capacity of trophoblasts. Target genes of miR-526b-5p were obtained by the dual luciferase reporter system. The promoter-reporter system and ChIP-qPCR were used to prove that c-Myc positively regulated the expression of Foxp1ResultsThe miR-526b-5p levels were significantly higher in patients with RSA than in controls. High expression of miR-526b-5p inhibited the proliferation, migration, and invasion of trophoblast cell line. By contrast, low expression of miR-526b-5p promoted the proliferation and migration of trophoblast cell line. Target genes of miR-526b-5p were c-Myc and Foxp1. c-Myc positively regulated the expression of Foxp1 by binding to the Foxp1 promoter location -146/-135. Finally, miR-526b-5p impeded the proliferation, migration, and invasion of trophoblasts by negatively regulating c-Myc by rescue experiments.ConclusionThus, miR-526b-5p affected the proliferation, migration, and invasion of trophoblasts by targeting c-Myc and Foxp1. Low expression of c-Myc further deactivated the positive transcriptional regulation of c-Myc on Foxp1, which may be the mechanism of RSA. This study provides potential therapeutic targets and clues for the diagnosis and treatment of RSA."

基金机构：Chongqing Postdoctoral Science Foundation [cstc2021jcyj-bsh0068]; Chongqing Postdoctoral Research Special Funding Project [2021XM3100]; National Natural Science Foundation of China [82171664]

基金资助正文："This study is supported by Chongqing Postdoctoral Science Foundation (cstc2021jcyj-bsh0068), Chongqing Postdoctoral Research Special Funding Project (2021XM3100), and the National Natural Science Foundation of China (82171664)."