CircPTPRA promotes the progression of pancreatic ductal adenocarcinoma via the miR-140-5p/LMNB1 axis

作者全名："Fu, Wen; Wang, Xianxing; Xiang, Jifeng; Chen, Shengkai; Xia, Renpei; Xie, Fuming; Chi, Bojing; Qin, Fanbo; Li, Zhuo; Mou, Li; Xie, Chuanming; Wang, Huaizhi"

作者地址："[Fu, Wen; Xie, Fuming; Qin, Fanbo; Wang, Huaizhi] Chongqing Med Univ, Chongqing 400016, Peoples R China; [Fu, Wen] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400174, Peoples R China; [Fu, Wen] Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400013, Peoples R China; [Fu, Wen; Wang, Xianxing; Xiang, Jifeng; Chen, Shengkai; Xia, Renpei; Wang, Huaizhi] Chongqing Gen Hosp, Dept Hepatopancreatobiliary Surg, Chongqing 401147, Peoples R China; [Chi, Bojing; Li, Zhuo] Chinese Acad Sci, Beijing 101400, Peoples R China; [Mou, Li] Chongqing Populat & Family Planning Sci & Technol, NHC Key Lab Birth Defects & Reprod Hlth, Chongqing 400020, Peoples R China; [Xie, Chuanming] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Key Lab Hepatobiliary & Pancreat Surg,Inst Hepatob, Chongqing 400038, Peoples R China"

通信作者："Wang, HZ (通讯作者)，Chongqing Med Univ, Chongqing 400016, Peoples R China.; Mou, L (通讯作者)，Chongqing Populat & Family Planning Sci & Technol, NHC Key Lab Birth Defects & Reprod Hlth, Chongqing 400020, Peoples R China.; Xie, CM (通讯作者)，Third Mil Med Univ, Army Med Univ, Southwest Hosp, Key Lab Hepatobiliary & Pancreat Surg,Inst Hepatob, Chongqing 400038, Peoples R China."

来源：CANCER MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000968326100001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：CircPTPRA; epithelial-mesenchymal transition; LMNB1; MiR-140-5p; pancreatic ductal adenocarcinoma

摘要："Background Growing evidences suggest that circular RNAs (circRNAs) are important factors in cancer progression. Nevertheless, the role of circRNAs in the progression of pancreatic ductal adenocarcinoma (PDAC) remains unclear.Methods CircPTPRA was identified based on our previous circRNA array data analysis. Wound healing, transwell, and EdU assays were performed to investigate the effect of circPTPRA on the migration, invasion, and proliferation of PDAC cells in vitro. RNA pull-down, fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP), and dual-luciferase reporter assays were conducted to verify the binding of circPTPRA with miR-140-5p. Subcutaneous xenograft model was constructed for in vivo experiment.Results CircPTPRA was significantly upregulated in PDAC tissues and cells compared to normal controls. Moreover, circPTPRA overexpression was positively correlated with lymph node invasion and worse prognosis in PDAC patients. In addition, overexpression of circPTPRA promoted PDAC migration, invasion, proliferation, and epithelial-mesenchymal transition (EMT) in vitro and in vivo. Mechanistically, circPTPRA upregulates LaminB1 (LMNB1) expression by sponging miR-140-5p and ultimately promotes the progression of PDAC.Conclusions This study revealed that circPTPRA plays an important role in the progression of PDAC by sponging miR-140-5p. It can be explored as a potential prognostic marker and therapeutic target for PDAC."

基金机构：Chongqing Science and Technology Commission [cstc2021ycjh-bgzxm0022]; National Natural Science Foundation of China [82072723]

基金资助正文：ACKNOWLEDGMENTS Thanks to Prof. Huaizhi Wang for his guidance on this project. Thanks to Prof. Chuanming Xie and Prof. Li Mou for providing the experimental platform. Thanks to the members of the lab for helpful discussions. This study was supported by the Chongqing Science and Technology Commission (No. cstc2021ycjh-bgzxm0022) and the National Natural Science Foundation of China (No. 82072723).