Electroacupuncture alleviates early brain injury via modulating microglia polarization and suppressing neuroinflammation in a rat model of subarachnoid hemorrhage

作者全名："Wang, Yingwen; Yang, Xiaomin; Cao, Yunchuan; Li, Xiaoguo; Xu, Rui; Yan, Jin; Guo, Zongduo; Sun, Shanquan; Sun, Xiaochuan; Wu, Yue"

作者地址："[Wang, Yingwen; Yang, Xiaomin; Cao, Yunchuan; Li, Xiaoguo; Xu, Rui; Yan, Jin; Guo, Zongduo; Sun, Xiaochuan; Wu, Yue] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China; [Sun, Shanquan] Chongqing Med Univ, Inst Neurosci, Chongqing, Peoples R China"

通信作者："Sun, XC; Wu, Y (通讯作者)，Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing, Peoples R China."

来源：HELIYON

ESI学科分类：　

WOS号：WOS:000970491300001

JCR分区：Q1

影响因子：3.4

年份：2023

卷号：9

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Electroacupuncture; Subarachnoid hemorrhage; Microglia polarization; Neuronal apoptosis; Neuroinflammation

摘要："Subarachnoid hemorrhage refers to an uncommon but severe subtype of stroke leading to high mortality and disability rates. Electroacupuncture, a traditional Chinese medical therapy com-bined with modern technology, shows evident curative effects on cerebral vascular diseases. This study attempts to investigate the possible treatment effects and mechanisms of EA on early brain injury after SAH. Data were gathered among sham group, SAH-induced group, and EA-treated group of male SD rats, concerning mortality rates, weight loss, rotarod latencies, cerebral blood flow, cell apoptosis, pro-inflammatory cytokines releasing, apoptotic protein level, microglia activation and related signal pathway. All results were collected 24-72 h after SAH induction. EA treatment demonstrated significant improvement on motor function 24 h after SAH without significant changes in mortality rate, weight loss, and cerebral blood flow. Another important finding was that EA regulated Bax and Bcl-2 imbalance and reduced cleaved casepase-3 caused by SAH. Additionally, levels of TNF-alpha, IL-1 beta, IL-6 were suppressed. The neuron apoptosis was suppressed by EA. The M1 polarization of activated microglia decreased while M2 polarized phenotype increased after EA treatment. Furthermore, pSTAT3-NOX2 signal axis, the M1 phenotype related activation pathway, was depressed after EA treatment. These findings sug-gested that EA improved motor deficits and ameliorated early brain injury after SAH probably via decreasing neuron apoptosis and anti-inflammation, which may involve modulation of microglia polarization. Taken together, EA may be a potential therapy for SAH treatment."

基金机构："National Natural Science Foundation of China [82071332, 82071397]; Chongqing Municipal Education Commission [KJQN202000428]; Chongqing Municipal Education Commission Foundation [CYS22342]"

基金资助正文："Dr. Yue Wu was supported by National Natural Science Foundation of China [81901210], Chongqing Municipal Education Commission [KJQN202000428]. Zongduo Guo and Xiaochuan Sun were supported by National Natural Science Foundation of China [82071332 & 82071397]. Yingwen Wang was supported by Chongqing Municipal Education Commission Foundation [CYS22342]."