Angelica Sinensis polysaccharide antagonizes 5-Fluorouracil-induced spleen injury and dysfunction by suppressing oxidative stress and apoptosis

作者全名："Du, Kunhang; Wang, Lu; Wang, Ziling; Xiao, Hanxianzhi; Hou, Jiying; Hu, Ling; Fan, Ningke; Wang, Yaping"

作者地址："[Du, Kunhang; Wang, Lu; Wang, Ziling; Xiao, Hanxianzhi; Hou, Jiying; Hu, Ling; Wang, Yaping] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China; [Fan, Ningke] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Wang, YP (通讯作者)，Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China."

来源：BIOMEDICINE & PHARMACOTHERAPY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000970566600001

JCR分区：Q1

影响因子：6.9

年份：2023

卷号：162

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Angelica polysaccharide (ASP); Spleen; Oxidative stress; Apoptosis

摘要："Angelica Sinensis polysaccharide (ASP), the main active component of Angelica sinensis, possesses antioxidative and anti-apoptotic properties. In this study, we have investigated the antagonistic effect of ASP on 5-FU-induced injury of mouse spleen in vivo and splenocytes in vitro, and its possible mechanism. Our results showed that ASP inhibited 5-FU-induced decreases in spleen weight and organ index in mice, restored the number of peripheral blood leukocytes and lymphocytes, repaired spleen structure disorder and functional impairment, rescued serum IL-2, IL-6, and IFN-gamma levels, and relieved 5-FU-induced mitochondrial swelling, reduced the oxidant accumu-lation including MDA and ROS, whereas increasing the activities of GSH, SOD and CAT. The mechanism may be related to ASP downregulation of Keap1 protein expression thus motivating the nuclear translocation of Nrf2. Furthermore, ASP alleviated the apoptosis of spleens in vivo and splenocytes in vitro, and reactivated PI3K / AKT signalling. In conclusion, the protective effect of ASP on spleens and splenocytes may be related to the reduction of oxidative stress and apoptosis via reactivation of Nrf2 and PI3K/AKT pathways. This study has provided a new protective agent for minimizing the spleen injury caused by 5-FU and a new idea for improving the prognosis of chemotherapy patients."

基金机构：National Natural Science Founda-tion of China [81873103]; General Program of Chongqing Natural Science Foundation [cstc2021jcyj-msxmX0669]

基金资助正文：Funding statement This study was supported by the National Natural Science Founda-tion of China (Nos. 81873103) and the General Program of Chongqing Natural Science Foundation (cstc2021jcyj-msxmX0669) .