RGD peptide modified RBC membrane functionalized biomimetic nanoparticles for thrombolytic therapy

作者全名："Xu, Zichen; Huang, Jinxia; Zhang, Tao; Xu, Wenfeng; Liao, Xiaoling; Wang, Yi; Wang, Guixue"

作者地址："[Xu, Zichen; Wang, Guixue] Bioengn Coll Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ, Chongqing 400030, Peoples R China; [Huang, Jinxia; Zhang, Tao; Xu, Wenfeng; Liao, Xiaoling] Chongqing Univ Sci & Technol, Sch Met & Mat Engn, Chongqing Key Lab Nano Micro Composite Mat & Devic, Chongqing 401331, Peoples R China; [Wang, Yi] Chongqing Med Univ, Coll Basic Med Sci, Chongqing 400016, Peoples R China"

通信作者："Wang, GX (通讯作者)，Bioengn Coll Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ, Chongqing 400030, Peoples R China.; Wang, Y (通讯作者)，Chongqing Med Univ, Coll Basic Med Sci, Chongqing 400016, Peoples R China."

来源：JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000970618700001

JCR分区：Q2

影响因子：4.2

年份：2023

卷号：34

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：Thrombus; Biomimetic; RBC membrane; RGD peptide; Targeted thrombolysis

摘要："In recent years, the fabrication of nano-drug delivery systems for targeted treatment of thrombus has become a research hotspot. In this study, we intend to construct a biomimetic nanomedicine for targeted thrombus treatment. The poly lactic-co-glycolic acid (PLGA) was selected as the nanocarrier material. Then, urokinase and perfluoro-n-pentane (PFP) were co-loaded into PLGA by the double emulsification solvent evaporation method to prepare phase change nanoparticles PPUNPs. Subsequently, the RGD peptide-modified red blood cell membrane (RBCM) was coated on the surface of PPUNPs to prepare a biomimetic nano-drug carrier (RGD-RBCM@PPUNPs). The as-prepared RGD-RBCM@PPUNPs possessed a ""core-shell"" structure, have good dispersibility, and inherited the membrane protein composition of RBCs. Under ultrasound stimulation, the loaded urokinase could be rapidly released. In vitro cell experiments showed that RGD-RBCM@PPUNPs had good hemocompatibility and cytocompatibility. Due to the coated RGD-RBC membrane, RGD-RBCM@PPUNPs could effectively inhibit the uptake of macrophages. In addition, RGD-RBCM@PPUNPs showed better thrombolytic function in vitro. Overall, the results suggested that this biomimetic nanomedicine provided a promising therapeutic strategy for the targeted therapy of thrombosis."

基金机构："National Natural Science Foundation of China [12032007, 31971242, 32201150]; Natural Science Foundation of Chongqing [cstc2019jcyj-zdxmX0028, cstc2017jcyjAX0186, CSTB2022NSCQ-MSX0096]; Chongqing Talents [cstc2022ycjh-bgzxm0166]; National ""111 Project"" Base [B0625]"

基金资助正文："Financial support from the National Natural Science Foundation of China (12032007, 31971242, 32201150), the Natural Science Foundation of Chongqing (cstc2019jcyj-zdxmX0028, cstc2017jcyjAX0186, CSTB2022NSCQ-MSX0096). and Chongqing Talents (cstc2022ycjh-bgzxm0166) as well as the National ""111 Project"" Base (B0625) are gratefully acknowledged. We gratefully thank the staff of the Public Experiment Centre of State Bioindustrial Base (Chongqing) for providing technical support and assistance in data collection and analysis."