Electroacupuncture of the cymba concha alleviates p-chlorophenylalanine-induced insomnia in mice

作者全名："Zhang, Fenyan; Zhang, Xiaoming; Peng, Qi; Tang, Ling"

作者地址："[Zhang, Fenyan; Tang, Ling] Chongqing Med Univ, Univ Town Hosp, Dept Neurol, 55 Middle Rd, Chongqing 401331, Peoples R China; [Zhang, Xiaoming] Hubei Univ Chinese Med, Coll Acupuncture & Orthoped, Wuhan, Peoples R China; [Zhang, Xiaoming] Hubei Univ Chinese Med, Subhlth Inst, Wuhan, Peoples R China; [Peng, Qi] Chongqing Med Univ, Univ Town Hosp, Chongqing, Peoples R China"

通信作者："Tang, L (通讯作者)，Chongqing Med Univ, Univ Town Hosp, Dept Neurol, 55 Middle Rd, Chongqing 401331, Peoples R China."

来源：ACUPUNCTURE IN MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000971557300001

JCR分区：Q2

影响因子：2.4

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：acupuncture; anxiety disorders; auricular acupuncture; electroacupuncture; internal medicine; neurology; psychiatry; sleep medicine

摘要："Objective: Insomnia is the most common sleep disorder and is often comorbid with mental and physical diseases. The present study was designed to investigate the hypnotic effect of electroacupuncture (EA) of the cymba concha to stimulate the auricular branch of the vagus nerve (ABVN). Methods: Mice were intraperitoneally injected with p-chlorophenylalanine (PCPA, 300 mg/kg center dot d) for 2 days to induce insomnia and subsequently received EA or manual acupuncture (MA) of the cymba concha for 30 min once daily for 5 consecutive days, or no treatment. The phenobarbital-induced sleep test was used to analyze the hypnotic effects and the open field test was used to analyze the locomotor activities and anxiolytic effects of EA/MA of the cymba concha. In addition, the levels of gamma-aminobutyric acid (GABA) and glutamate (Glu) in the hypothalamus and peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA). Results: PCPA injection significantly decreased sleep duration, increased sleep latency and induced anxiety-like behaviors in mice. In PCPA-insulted mice, EA of the cymba concha improved the sleep disturbance by significantly prolonging sleep duration, while no change in sleep latency was observed. Moreover, EA of the cymba concha improved PCPA-induced anxiety-like behaviors without decreasing locomotor activities in the open field test. EA of the cymba concha increased the level of GABA in the hypothalamus and peripheral blood, while Glu concentrations remained unchanged. Conclusion: These findings indicate that EA of the region innervated by the ABVN upregulates GABA levels in the hypothalamus and ameliorates the symptoms of insomnia and anxiety, suggesting that EA of the cymba concha might have potential value as an intervention for insomnia."

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