"Profile of Human Milk Phospholipids at Different Lactation Stages with UPLC/Q-TOF-MS: Characterization, Distribution, and Differences"

作者全名："Ding, Desheng; He, Xiaoling; Agarry, Israel Emiezi; Wang, Yuankai; Zhou, Fenglan; Li, Yunchang; Kan, Jianquan; Cai, Tian; Chen, Kewei"

作者地址："[Ding, Desheng; Agarry, Israel Emiezi; Wang, Yuankai; Zhou, Fenglan; Li, Yunchang; Kan, Jianquan; Chen, Kewei] Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China; [Ding, Desheng; Agarry, Israel Emiezi; Wang, Yuankai; Zhou, Fenglan; Li, Yunchang; Kan, Jianquan; Chen, Kewei] Chongqing Key Lab Specialty Food Cobuilt Sichuan &, Chongqing 400715, Peoples R China; [He, Xiaoling] Chongqing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Agarry, Israel Emiezi; Kan, Jianquan; Chen, Kewei] Chinese Hungarian Cooperat Res Ctr Food Sci, Chongqing 400715, Peoples R China; [Cai, Tian] Southwest Univ, Sch Chem & Chem Engn, Chongqing 400715, Peoples R China"

通信作者："Chen, KW (通讯作者)，Chongqing Key Lab Specialty Food Cobuilt Sichuan &, Chongqing 400715, Peoples R China.; Chen, KW (通讯作者)，Chinese Hungarian Cooperat Res Ctr Food Sci, Chongqing 400715, Peoples R China.; Cai, T (通讯作者)，Southwest Univ, Sch Chem & Chem Engn, Chongqing 400715, Peoples R China."

来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ESI学科分类：AGRICULTURAL SCIENCES

WOS号：WOS:000971965200001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：71

期号：16

开始页：6326

结束页：6337

文献类型：Article

关键词：human milk; glycerophospholipid; sphingomyelin; lactation stage; UPLC; Q-TOF-MS

摘要："Human milk phospholipids are important for the regular growth and development of infants. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was employed to qualitatively and quantitatively analyze 277 phospholipid molecular species in 112 human milk samples to obtain a detailed profile of human milk phospholipids along the lactation stage. MS/MS fragmentation patterns of sphingomyelin, phosphatidylcholine, phosphatidyletha-nolamine, phosphatidylinositol, and phosphatidylserine were characterized in detail. Phosphatidylcholine is the most dominant group, followed by sphingomyelin. PC(18:0/18:2), SM(d18:1/24:1), PE(18:0/18:0), PS(18:0/20:4), and PI(18:0/18:2) showed the highest average concentration among all of the phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol molecular species, respectively. The fatty acids attached to the phospholipid molecules were mainly palmitic, stearic, oleic, and linoleic acids, and the plasmalogens decreased along the lactation stage. The increase of sphingomyelins and phosphatidylethanolamines and the decrease of phosphatidylcholines are the key changes from colostrum to transitional milk; the increase of lysophosphatidylcholines and lysophosphatidylethanolamines and the continuous decrease of phosphatidylcholines are the vital changes from transitional milk to mature milk."

基金机构：Chinese Institute of Food Science and Technology [2020-03]; Southwest University [SWU019025]; project from Key Project of Innovation Research 2035 Pilot Plan of Southwest University; [SWU-XDZD22007]

基金资助正文："This work was supported by the project from the Chinese Institute of Food Science and Technology (2020-03) , Southwest University (SWU019025) , and the project from Key Project of Innovation Research 2035 Pilot Plan of Southwest University (SWU-XDZD22007) ."