MicroRNA Imaging Encounters Rolling Circle Amplification: Intracellular Na plus -Fueled Linear Programmable DNAzyme Nanostructure

作者全名："Shuai, Xinjia; Zhang, Yue; Zhai, Jia; Li, Jing; Chen, Jiao; Long, Xinying; Li, Di; Huang, Chengzhi; Li, Chunmei"

作者地址："[Shuai, Xinjia; Zhang, Yue; Zhai, Jia; Li, Jing; Chen, Jiao; Long, Xinying; Huang, Chengzhi; Li, Chunmei] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China; [Li, Di] Chongqing Med Univ, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Li, CM (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing 400715, Peoples R China."

来源：ANALYTICAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000971981500001

JCR分区：Q1

影响因子：6.7

年份：2023

卷号：95

期号：16

开始页：6681

结束页：6689

文献类型：Article

关键词：　

摘要："DNAzyme motors are widely used for the sensitive detection of intracellular miRNAs due to their excellent signal response. Generally, the addition of exogenous mental ions to DNAzyme motors is crucial for the efficient operation of the system. Moreover, the position of the DNAzyme relative to the substrate has a significant impact on the cleavage rate during the reaction. Herein, we proposed a highly loaded Na+-fueled linear programmable DNAzyme nanostructure (LPDN) composed of long, single-strand DNA produced by rolling circle amplification reactions that served as binding partners for Na+- specific DNAyme and substrate. In the meantime, the long, programmable scaffolds can precisely control the position of the DNAzyme and substrate for the optimal effect. During the assay, miR-21 and endogenous Na+ can specifically trigger multiple adjacent substrate-cleaving reactions, resulting in a significant recovery of the Cy3 fluorescence signal in living cells. This method could enable in situ real-time imaging and biocompatibility-enhancing evaluation of intracellular miR-21-level changes. Furthermore, LPDN's ability to distinguish normal cells from cancer cells makes it a promising candidate for early cancer diagnosis and imaging analysis of cancer."

基金机构：National Natural Science Foundation of China (NSFC) [22074124]; Natural Science Foundation of Chongqing [CSTB2022NSCQ-MSX0521]; fund of Fundamental Research Funds for the Central Universities [XDJK2020TY001]; Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates [S202210635385]

基金资助正文："This work was financially supported by the National Natural Science Foundation of China (NSFC, no. 22074124), Natural Science Foundation of Chongqing (CSTB2022NSCQ-MSX0521), and the fund of Fundamental Research Funds for the Central Universities (XDJK2020TY001), as well as the Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates (S202210635385). The authors also thank Prof. Dayong Yang at School of Chemical Engineering and Technology in Tianjin University for providing Phi29 DNA polymerase for this work."