Chrysin and its nanoliposome ameliorated non-alcoholic steatohepatitis via inhibiting TLR4 signalling pathway

作者全名："Liu, Hao; Jiang, Ningman; Kuang, Ge; Gong, Xia; Hu, Jun; Liu, Jin; Yin, Xinru; Wu, Shengwang; Wan, Jingyuan"

作者地址："[Liu, Hao; Jiang, Ningman; Kuang, Ge; Hu, Jun; Liu, Jin; Wan, Jingyuan] Chongqing Med Univ, Dept Pharmacol, Chongqing Key Lab Biochem & Mol Pharmacol, Chongqing, Peoples R China; [Gong, Xia] Chongqing Med Univ, Dept Anat, Chongqing, Peoples R China; [Yin, Xinru] Army Med Univ, Daping Hosp, Inst Surg Res, Dept Gastroenterol, Chongqing, Peoples R China; [Wu, Shengwang] Army Med Univ, Xinqiao Hosp, Dept Hematol, Chongqing, Peoples R China; [Wan, Jingyuan] Chongqing Med Univ, Dept Pharmacol, 1 Med Coll Rd, Chongqing, Yuzhong, Peoples R China"

通信作者："Wan, JY (通讯作者)，Chongqing Med Univ, Dept Pharmacol, 1 Med Coll Rd, Chongqing, Yuzhong, Peoples R China."

来源：JOURNAL OF PHARMACY AND PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000972048100001

JCR分区：Q2

影响因子：2.8

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：chrysin; nanoliposome; non-alcoholic steatohepatitis; Toll-like receptor 4

摘要："Objectives Non-alcoholic steatohepatitis (NASH) is a chronic liver disease histologically characterized by liver steatosis, hepatocellular injury, inflammation and fibrosis, resulting in cirrhosis and hepatocellular carcinoma, but effective measures and obvious pathogenesis for NASH remain elusive. Chrysin (CH) has been reported to have anti-inflammatory effects but shows lower bioavailability.Methods In this study, a chrysin nanoliposome (CH-NL) was first prepared and characterized. Then, we used the methionine-choline-deficient (MCD) diet to induce a mouse model of NASH. Finally, the effects of CH and CH-NL on NASH were evaluated in the liver of NASH mice.Key findings The results showed that CH or CH-NL significantly reduced the accumulation of lipids in hepatocytes, alleviated liver injury, decreased the generation of radical oxygen species, and attenuated the accumulation of collagen fibre in the liver of NASH mice. In addition, CH and its nano-liposomes markedly inhibited the production of inflammatory cytokines and inflammatory cell infiltration in the liver of NASH mice. Further studies found that CH-NL and CH-NL downregulated the MCD diet-induced activation of Toll-like receptor 4 (TLR4) signalling pathway in the liver of mice.Conclusions CH and its nanoliposome alleviated MCD diet-induced NASH in mice, which might be through inhibiting TLR4 signalling pathway."

基金机构：Natural Science Foundation of China [81600455]

基金资助正文："This work supported by the grant from the Natural Science Foundation of China, grant number 81600455."