DNA-Guided Extracellular-Vesicle Metallization with High Catalytic Activity for Accurate Diagnosis of Pulmonary Nodules

作者全名："Chen, Yirong; Zhang, Songzhi; Lu, Jun; Li, Dandan; Wu, Haiping; Zhang, Lu; Li, Xinyu; Gao, Xin; Xu, Yuan; Zeng, Zijie; Zeng, Li; Ding, Xiaojuan; Li, Xinmin; Ding, Shijia"

作者地址："[Chen, Yirong; Zhang, Songzhi; Lu, Jun; Wu, Haiping; Zhang, Lu; Li, Xinyu; Gao, Xin; Xu, Yuan; Zeng, Zijie; Li, Xinmin; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Li, Dandan; Ding, Xiaojuan] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 2, Chongqing 400016, Peoples R China; [Zeng, Li] Chongqing Tradit Chinese Med Hosp, Dept Lab Med, Chongqing 400016, Peoples R China"

通信作者："Li, XM; Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：SMALL

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000973334900001

JCR分区：Q1

影响因子：13

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：colorimetric assay; DNA-guided metallization; extracellular vesicles; pulmonary nodules

摘要："Sensitive and specific analysis of extracellular vesicles (EVs) offers a promising minimally invasive way to identify malignant pulmonary nodules from benign lesions. However, accurate analysis of EVs is subject to free target proteins in blood samples, which compromises the clinical diagnosis value of EVs. Here a DNA-guided extracellular-vesicle metallization (DEVM) strategy is described for ultrasensitive and specific analysis of EV protein biomarkers and classification of pulmonary nodules. The facile DEVM process mainly includes the incorporation of DNA labeled with cholesterol and thiol groups into EV membranes and subsequent deposition of Au3+ and Pt4+ to allow the DNA-functionalized EVs to be encapsulated with AuPt nanoshells. It is found that the synthesized AuPt-metallized EVs possess extrinsic peroxidase-like activity. Utilizing the feature of the catalytic metal nanoshells just growth on the EV membranes, the DEVM method enables multiparametric recognition of target proteins and EV membranes and can produce an amplified colorimetric signal, avoiding the interference of free proteins. By profiling four surface proteins of EVs from 48 patients with pulmonary nodules, the highest area under the receiver operating characteristic curve (0.9983) is obtained. Therefore, this work provides a feasible EVs analysis tool for accurate pulmonary nodules management."

基金机构："National Natural Science Foundation of China [81873980, 82202649, 81902164]; Chongqing Talent-Innovation Leader Project [cstc2022ycjh-bgzxm0001]; Chongqing Basic Frontier Research [cstc2020jcyj-msxmX0190]; Chongqing Medical Scientific Research Project-Joint project of Chongqing Health Commission and Science and Technology Bureau [2022MSXM008]; Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0179]; Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University [kryc-yq-2117, kryc-yq-2125]"

基金资助正文："Y.C., S.Z., J.L., and D.L. contributed equally to this work. This work was carried out according to the guidelines approved by the ethics committees from the institutions involved. Informed consents were obtained from all participants of this clinical study, clinical samples were obtained after approval of the institutional review board at Chongqing Traditional Chinese Medicine Hospital, and all experiments were performed in accordance with the approved guidelines and regulations by the ethics committee at Chongqing Medical University. This work was supported by the National Natural Science Foundation of China (81873980, 82202649, and 81902164), the Chongqing Talent-Innovation Leader Project (cstc2022ycjh-bgzxm0001), Chongqing Basic Frontier Research (cstc2020jcyj-msxmX0190), Chongqing Medical Scientific Research Project-Joint project of Chongqing Health Commission and Science and Technology Bureau (2022MSXM008), Natural Science Foundation of Chongqing, China (cstc2019jcyj-msxmX0179), and Kuanren Talents Program of the second affiliated hospital of Chongqing Medical University (kryc-yq-2117 and kryc-yq-2125)."