Geiparvarin Inhibits the Progression of Osteosarcoma by Down-regulating COX2 Expression

作者全名："Wang, Bin; Du, Jia; Zhang, Zhiming; Huang, Ping; Chen, Shu; Zou, Hua"

作者地址："[Wang, Bin; Du, Jia; Huang, Ping; Chen, Shu; Zou, Hua] Army Med Univ, Daping Hosp, Dept Oncol, Chongqing 400042, Peoples R China; [Wang, Bin] Chongqing Med Univ, Dept Cell Biol & Genet, Chongqing 400016, Peoples R China; [Zhang, Zhiming] Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Thorac Surg, 69 Yongding Rd, Beijing 100089, Peoples R China; [Chen, Shu; Zou, Hua] Army Med Univ, Daping Hosp, Dept Oncol, 10 Daping Changjiang Branch Rd, Chongqing 400042, Peoples R China"

通信作者："Chen, S; Zou, H (通讯作者)，Army Med Univ, Daping Hosp, Dept Oncol, 10 Daping Changjiang Branch Rd, Chongqing 400042, Peoples R China."

来源：CURRENT CANCER DRUG TARGETS

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000973749800005

JCR分区：Q3

影响因子：2.3

年份：2023

卷号：23

期号：5

开始页：379

结束页：387

文献类型：Article

关键词：Geiparvarin; osteosarcoma; COX2; angiogenesis; metastasis; apoptosis

摘要："Background: Geiparvarin (GN) is a natural compound isolated from the leaves of Geijera parviflora and exhibits anticancer activity. Nevertheless, little is known about its anticancer mechanism and anti-osteosarcoma (OS) effects.Aim: This study explored whether GN effectively inhibits the growth and metastasis of osteosarcoma (OS) through a series of in vitro and in vivo experiments.Methods: Cell proliferation was measured by colony formation and MTT assays, and cell invasion was detected by Transwell assay. Flow cytometry and caspase-3 activity assays were carried out to examine cell apoptosis, and western blot analysis was performed to assess protein expression. In the animal experiments, the changes in relevant indexes were determined by immunohistochemistry and tumor vessel imaging.Results: Animal experiments showed that GN treatment significantly inhibited the growth and lung metastasis of OS, accompanied by increased apoptosis. In addition, GN treatment notably diminished COX2 expression and angiogenesis in OS. Moreover, COX2 overexpression nullified GN-induced decline in angiogenesis, growth, and lung metastasis and increased apoptosis in OS. Of note, the body weight of mice was enhanced after GN treatment, and the pathological examination manifested that GN treatment did not cause any damage to major organs.Conclusion: Our data indicated that GN might depress the growth, metastasis, and angiogenesis of OS by decreasing COX2 expression, suggesting GN is a favorable candidate drug for OS treatment without side effects. Hence, it can be concluded that geiparvarin inhibits OS progression by reducing COX2 expression."

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