Rg1 alleviates oxidative stress and spermatogonium apoptosis in D-gal-induced testicular toxicity by activating Akt

作者全名："Wang, Ziling; Du, Kunhang; Hou, Jiying; Xiao, Hanxianzhi; Hu, Ling; Chen, Xiongbin; Wang, Lu; Wang, Yaping"

作者地址："[Wang, Ziling; Du, Kunhang; Hou, Jiying; Xiao, Hanxianzhi; Hu, Ling; Wang, Lu; Wang, Yaping] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing, Peoples R China; [Chen, Xiongbin] Chengdu Univ Tradit Chinese Med, Basic Med Coll, Dept Anat & Histol & Embryol, Chengdu, Sichuan, Peoples R China; [Wang, Yaping] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China"

通信作者："Wang, YP (通讯作者)，Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China."

来源：REDOX REPORT

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000975671800001

JCR分区：Q1

影响因子：5.2

年份：2023

卷号：28

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Ginsenoside Rg1; testis; oxidative damage; apoptosis

摘要："Objectives: High reactive oxygen species (ROS) levels lead to cell death, and the testes are among the most vulnerable organs to oxidative damage. Rg1, an active ingredient extracted from the natural medicine ginseng, has potential anti-inflammatory, antioxidant and antiapoptotic properties. Our previous studies showed that Rg1 can effectively improve spermatogenic function in mice, but the specific mechanism remains unclear. The purpose of this study was to investigate the effect of Rg1 on oxidative stress and spermatogonium apoptosis in D-gal-induced testicular toxicity and elucidate the associated mechanism. Methods: Male C57BL/6 mice at 6-8 weeks of age were intraperitoneally injected with D-gal (200 mg/kg) for 42 days to establish a testicular injury model, and on day 16, 40 mg/kg Rg1-rich saline was injected intraperitoneally. Concurrently, we established an in vitro model of D-gal-damaged spermatogonia, which was treated with Rg1. Results: We found that treatment with the ginsenoside Rg1 reduced D-gal-induced oxidative stress and spermatogonium apoptosis in vivo and in vitro. Mechanistically, we found that Rg1 activated Akt/bad signaling and reduced D-gal-induced spermatogonium apoptosis. Discussion: We provide evidence showing that the antioxidant effect of Rg1 is mediated by the Akt/GSK-3 beta/NRF2 axis. Based on these findings, we consider Rg1 a potential treatment for testicular oxidative damage."

基金机构：National Natural Science Foundation of China [81873103]

基金资助正文：This study was supported by the National Natural Science Foundation of China (no. 81873103).