Tropoelastin-Pretreated Exosomes from Adipose-Derived Stem Cells Improve the Synthesis of Cartilage Matrix and Alleviate Osteoarthritis

作者全名："Meng, Shuo; Tang, Cong; Deng, Muhai; Yuan, Jie; Fan, Yanli; Gao, Shasha; Feng, Yong; Yang, Junjun; Chen, Cheng"

作者地址："[Meng, Shuo; Tang, Cong; Deng, Muhai; Yuan, Jie; Fan, Yanli; Gao, Shasha; Chen, Cheng] Chongqing Med Univ, Coll Med Informat, Chongqing 400016, Peoples R China; [Feng, Yong] Chongqing Univ, Chongqing Emergency Med Ctr, Dept Orthopaed Surg, Cent Hosp, Chongqing 400014, Peoples R China; [Yang, Junjun] Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China"

通信作者："Chen, C (通讯作者)，Chongqing Med Univ, Coll Med Informat, Chongqing 400016, Peoples R China.; Yang, JJ (通讯作者)，Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China."

来源：JOURNAL OF FUNCTIONAL BIOMATERIALS

ESI学科分类：　

WOS号：WOS:000977360100001

JCR分区：Q1

影响因子：5

年份：2023

卷号：14

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：tropoelastin; adipose-derived stem cells; exosomes; articular cartilage; osteoarthritis

摘要："Mesenchymal stem cells (MSCs) have recently been widely used to treat osteoarthritis (OA). Our prior research shows that tropoelastin (TE) increases MSC activity and protects knee cartilage from OA-related degradation. The underlying mechanism might be that TE regulates the paracrine of MSCs. Exosomes (Exos), the paracrine secretion of MSCs, have been found to protect chondrocytes, reduce inflammation, and preserve the cartilage matrix. In this study, we used Exos derived from TE-pretreated adipose-derived stem cells (ADSCs) (TE-Exo(ADSCs)) as an injection medium, and compared it with Exos derived from unpretreated ADSCs (Exo(ADSCs)). We found that TE-Exo(ADSCs) could effectively enhance the matrix synthesis of chondrocytes in vitro. Moreover, TE pretreatment increased the ability of ADSCs to secrete Exos. In addition, compared with Exo(ADSCs), TE-Exo(ADSCs) exhibited therapeutic effects in the anterior cruciate ligament transection (ACLT)-induced OA model. Further, we observed that TE altered the microRNA expression in Exo(ADSCs) and identified one differentially upregulated microRNA: miR-451-5p. In conclusion, TE-Exo(ADSCs) helped maintain the chondrocyte phenotype in vitro, and promoted cartilage repair in vivo. These therapeutic effects might be related with the altered expression of miR-451-5p in the Exo(ADSCs). Thus, the intra-articular delivery of Exos derived from ADSCs with TE pretreatment could be a new approach to treat OA."

基金机构：Science and Technology Projects of Chongqing Education Commission [KJQN202000427]; Natural Science Foundation of Chongqing [cstc2020jcyj-msxmX0585]; Future Medical Innovation Team of Chongqing Medical University [W0080]; Student Scientific Research and Innovation Experiment Project of the School of Medical Information [2020C003]; Project of Innovative Science Research for Postgraduates of Chongqing Municipal Education Committee [CYS21251]

基金资助正文："This research was funded by the Science and Technology Projects of Chongqing Education Commission (KJQN202000427), the Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0585), the Future Medical Innovation Team of Chongqing Medical University (W0080), the Student Scientific Research and Innovation Experiment Project of the School of Medical Information (2020C003), and the Project of Innovative Science Research for Postgraduates of Chongqing Municipal Education Committee (CYS21251)."